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PMID: 17138700 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The POLARIS peptide of Arabidopsis regulates auxin transport and root growth via effects on ethylene signaling.

The Plant cell ·Vol. 18 ·No. 11 ·2006-11-00 ·Pages 3058-72

Chilley PM, Casson SA, Tarkowski P, Hawkins N, Wang KL, Hussey PJ, Beale M, Ecker JR, Sandberg GK, Lindsey K

Abstract

The rate and plane of cell division and anisotropic cell growth are critical for plant development and are regulated by diverse mechanisms involving several hormone signaling pathways. Little is known about peptide signaling in plant growth; however, Arabidopsis thaliana POLARIS (PLS), encoding a 36-amino acid peptide, is required for correct root growth and vascular development. Mutational analysis implicates a role for the peptide in hormone responses, but the basis of PLS action is obscure. Using the Arabidopsis root as a model to study PLS action in plant development, we discovered a link between PLS, ethylene signaling, auxin homeostasis, and microtubule cytoskeleton dynamics. Mutation of PLS results in an enhanced ethylene-response phenotype, defective auxin transport and homeostasis, and altered microtubule sensitivity to inhibitors. These defects, along with the short-root phenotype, are suppressed by genetic and pharmacological inhibition of ethylene action. PLS expression is repressed by ethylene and induced by auxin. Our results suggest a mechanism whereby PLS negatively regulates ethylene responses to modulate cell division and expansion via downstream effects on microtubule cytoskeleton dynamics and auxin signaling, thereby influencing root growth and lateral root development. This mechanism involves a regulatory loop of auxin-ethylene interactions.

MeSH Terms
Amino Acids, Cyclic/pharmacology Arabidopsis/drug effects,growth & development,metabolism Arabidopsis Proteins/genetics,metabolism Biological Transport/drug effects Ethylenes/metabolism Genes, Plant Indoleacetic Acids/metabolism Models, Biological Molecular Sequence Data Mutation/genetics Peptides/metabolism Phenotype Plant Roots/cytology,drug effects,growth & development Signal Transduction/drug effects Transcription, Genetic/drug effects Tubulin/metabolism
Chemicals
Amino Acids, Cyclic Arabidopsis Proteins Ethylenes Indoleacetic Acids Peptides Tubulin polaris protein, Arabidopsis 1-aminocyclopropane-1-carboxylic acid ethylene
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Chilley Paul M
Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, Durham University, Durham DH1 3LE, UK.
Casson Stuart A
Tarkowski Petr
Hawkins Nathan
Wang Kevin L-C
Hussey Patrick J
Beale Mike
Ecker Joseph R
Sandberg Göran K
Lindsey Keith
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.106.040790
Published
2006-11-00
Epub
2006-00-30
Pages
3058-72
Language
English
Region
England
NLM ID
9208688
PMCID
PMC1693943
Subset
IM
Grants
Biotechnology and Biological Sciences Research Council · BB/E006531/1 · United Kingdom
Databases
GENBANK
AF285768
External Links
PubMed source DOI: 10.1105/tpc.106.040790 PMC Full Text View journal details
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