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PMID: 17158804 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural

Polycomb recruitment to DNA in vivo by the YY1 REPO domain.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 103 ·No. 51 ·2006-12-19 ·Pages 19296-301

Wilkinson FH, Park K, Atchison ML

Abstract

Polycomb group (PcG) proteins are responsible for maintaining transcriptional repression of developmentally important genes. However, the mechanism of PcG recruitment to specific DNA sequences is poorly understood. Transcription factor YY1 is one of the few PcG proteins with sequence-specific DNA binding activity. We previously showed that YY1 can recruit other PcG proteins to DNA, leading to histone posttranslational modifications and stable transcriptional repression. Using Drosophila transgenic approaches, we identified YY1 sequences 201-226 as necessary and sufficient for PcG transcriptional repression in vivo. When fused to a heterologous DNA-binding domain, this short 26-aa motif was sufficient for transcriptional repression, recruitment of PcG proteins to DNA, and methylation of histone H3 lysine 27. Deletion of this short YY1 motif did not affect transient transcriptional repression but ablated PcG repression, PcG protein recruitment to DNA, and methylation of H3 lysine 27. We propose that this motif be named the REPO domain for its function in recruitment of Polycomb. The REPO domain is well conserved in YY1 orthologs and in related proteins.

MeSH Terms
Animals Chromatin Immunoprecipitation DNA/metabolism DNA Primers Drosophila/genetics Drosophila Proteins/metabolism Gene Expression Regulation/genetics Histones/metabolism Mice NIH 3T3 Cells Polycomb Repressive Complex 1 Protein Structure, Tertiary Transfection YY1 Transcription Factor/genetics,metabolism
Chemicals
DNA Primers Drosophila Proteins Histones Pc protein, Drosophila YY1 Transcription Factor DNA Polycomb Repressive Complex 1
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wilkinson Frank H
Department of Animal Biology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA 19104, USA.
Park Kyoungsook
Atchison Michael L
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44 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0603564103
Published
2006-12-19
Epub
2006-00-08
Pages
19296-301
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC1748220
Subset
IM
Grants
NIGMS NIH HHS · R01 GM042415 · United States
NIDDK NIH HHS · T32DK07708 · United States
NIGMS NIH HHS · R01 GM071830 · United States
NIGMS NIH HHS · GM71830 · United States
NIDDK NIH HHS · T32 DK007708 · United States
NIGMS NIH HHS · GM42415 · United States
External Links
PubMed source DOI: 10.1073/pnas.0603564103 PMC Full Text View journal details
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