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PMID: 1717477 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Paxillin is a major phosphotyrosine-containing protein during embryonic development.

The Journal of cell biology ·Vol. 115 ·No. 1 ·1991-10-00 ·Pages 201-7

Turner CE

Abstract

Phosphotyrosine-containing proteins were immunoprecipitated from embryonic chicken tissue extracts using anti-phosphotyrosine antibody coupled to agarose beads. Major phosphotyrosine-containing proteins of 110, 70, and 50 kD were observed following blotting with anti-phosphotyrosine antibody. The 70-kD band was selectively removed from the samples by precipitation with antibodies to the focal adhesion protein paxillin, therefore identifying paxillin as one of the major tyrosine kinase substrates during chick embryonic organogenesis. The tyrosine phosphorylation of paxillin is regulated developmentally: during embryogenesis, a marked decrease in its phosphotyrosine content was observed, although the total level of paxillin remained essentially constant. Approximately 20% of the paxillin was phosphorylated on tyrosine in the early embryo. In contrast, tyrosine phosphorylation of paxillin was undetectable in the adult. A similar profile of phosphotyrosine-containing proteins was identified in rat embryos. Paxillin was also found to be a major phosphotyrosine-containing protein in the rat embryo. These data suggest that the regulated phosphorylation of tyrosine residues on paxillin may perform a critical role in controlling cell and tissue cytoarchitecture rearrangement during vertebrate development.

MeSH Terms
Animals Chick Embryo Cytoskeletal Proteins/chemistry,immunology,metabolism Molecular Weight Paxillin Phosphoproteins/chemistry,immunology,metabolism Phosphotyrosine Precipitin Tests Tissue Distribution Tyrosine/analogs & derivatives,metabolism
Chemicals
Cytoskeletal Proteins Paxillin Phosphoproteins Phosphotyrosine Tyrosine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Turner C E
Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599-7090.
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43 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.115.1.201
Published
1991-10-00
Pages
201-7
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289923
Subset
IM
Grants
NIGMS NIH HHS · GM 29860 · United States
NIGMS NIH HHS · GM47607 · United States
External Links
PubMed source DOI: 10.1083/jcb.115.1.201 PMC Full Text View journal details
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