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PMID: 17220199 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The PEROXIN11 protein family controls peroxisome proliferation in Arabidopsis.

The Plant cell ·Vol. 19 ·No. 1 ·2007-01-00 ·Pages 333-50

Orth T, Reumann S, Zhang X, Fan J, Wenzel D, Quan S, Hu J

Abstract

PEROXIN11 (PEX11) is a peroxisomal membrane protein in fungi and mammals and was proposed to play a major role in peroxisome proliferation. To begin understanding how peroxisomes proliferate in plants and how changes in peroxisome abundance affect plant development, we characterized the extended Arabidopsis thaliana PEX11 protein family, consisting of the three phylogenetically distinct subfamilies PEX11a, PEX11b, and PEX11c to PEX11e. All five Arabidopsis PEX11 proteins target to peroxisomes, as demonstrated for endogenous and cyan fluorescent protein fusion proteins by fluorescence microscopy and immunobiochemical analysis using highly purified leaf peroxisomes. PEX11a and PEX11c to PEX11e behave as integral proteins of the peroxisome membrane. Overexpression of At PEX11 genes in Arabidopsis induced peroxisome proliferation, whereas reduction in gene expression decreased peroxisome abundance. PEX11c and PEX11e, but not PEX11a, PEX11b, and PEX11d, complemented to significant degrees the growth phenotype of the Saccharomyces cerevisiae pex11 null mutant on oleic acid. Heterologous expression of PEX11e in the yeast mutant increased the number and reduced the size of the peroxisomes. We conclude that all five Arabidopsis PEX11 proteins promote peroxisome proliferation and that individual family members play specific roles in distinct peroxisomal subtypes and environmental conditions and possibly in different steps of peroxisome proliferation.

MeSH Terms
Arabidopsis/growth & development,metabolism,ultrastructure Arabidopsis Proteins/analysis,genetics,physiology Genetic Complementation Test Intracellular Membranes/metabolism,ultrastructure Luminescent Proteins/analysis Membrane Proteins/analysis,genetics,physiology Molecular Sequence Data Multigene Family/physiology Peroxins Peroxisomes/metabolism,ultrastructure Phenotype Phylogeny Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins/genetics
Chemicals
Arabidopsis Proteins Luminescent Proteins Membrane Proteins PEX11 protein, S cerevisiae Peroxins Saccharomyces cerevisiae Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Orth Travis
Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824, USA.
Reumann Sigrun
Zhang Xinchun
Fan Jilian
Wenzel Dirk
Quan Sheng
Hu Jianping
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.106.045831
Published
2007-01-00
Epub
2007-00-12
Pages
333-50
Language
English
Region
England
NLM ID
9208688
PMCID
PMC1820951
Subset
IM
Databases
GENBANK
AAF75750, ABF93659, ABF95492, ABF95493, BAD01558, BAD67925, CAD41517, CAG13099, CAG81724, EAA31192, EAA65086, O96011
RefSeq
NP_003838, NP_014494, NP_014836, NP_015213, NP_190327, NP_191666, NP_563636, NP_564514, NP_850441, XP_545854
External Links
PubMed source DOI: 10.1105/tpc.106.045831 PMC Full Text View journal details
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