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PMID: 1732205 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Lysyl-tRNA synthetase gene of Campylobacter jejuni.

Journal of bacteriology ·Vol. 174 ·No. 3 ·1992-02-00 ·Pages 695-701

Chan VL, Bingham HL

Abstract

We report the cloning and complete nucleotide sequence of the Campylobacter jejuni lysyl-tRNA synthetase gene (lysS). The C. jejuni lysS gene sequence shows high homology to the two Escherichia coli lysyl-tRNA synthetase genes, lysS and lysU. The Campylobacter lysyl-tRNA synthetase protein (LysRS) shows 47.9 and 46.6% sequence identity to the E. coli enzymes encoded by the lysS and lysU genes, respectively. The LysRS encoded by the C. jejuni gene is a polypeptide of 501 amino acids with a deduced molecular weight of 57,867. The enzyme is active in E. coli. The gene is expressed from its own promoter, and the transcription start site has been mapped. The carboxyl-terminal codon of the C. jejuni lysS gene overlaps by 1 bp with the Met initiation codon of the glyA gene, which has been shown to have a promoter which is functional in E. coli (V.L. Chan and H.L. Bingham, Gene 101:51-58, 1991). C. jejuni, unlike E. coli, has only one lysyl-tRNA synthetase gene.

Related Genes
lysS
MeSH Terms
Amino Acid Sequence Base Sequence Campylobacter/genetics Campylobacter jejuni/genetics Cloning, Molecular Escherichia coli/genetics Gene Expression Genetic Complementation Test Lysine-tRNA Ligase/genetics Molecular Sequence Data Restriction Mapping Saccharomyces cerevisiae/genetics Sequence Homology, Nucleic Acid Transcription, Genetic
Chemicals
Lysine-tRNA Ligase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chan V L
Department of Microbiology, University of Toronto, Ontario, Canada.
Bingham H L
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28 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.3.695-701.1992
Published
1992-02-00
Pages
695-701
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206145
Subset
IM
Databases
GENBANK
L01134, L01135, L01136, L01137, L01138, L01139, L01140, L01141, M63448, M94104
External Links
PubMed source DOI: 10.1128/jb.174.3.695-701.1992 PMC Full Text View journal details
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