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PMID: 17337521 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of lipid rafts from Medicago truncatula root plasma membranes: a proteomic study reveals the presence of a raft-associated redox system.

Plant physiology ·Vol. 144 ·No. 1 ·2007-05-00 ·Pages 402-18

Lefebvre B, Furt F, Hartmann MA, Michaelson LV, Carde JP, Sargueil-Boiron F, Rossignol M, Napier JA, Cullimore J, Bessoule JJ, Mongrand S

Abstract

Several studies have provided new insights into the role of sphingolipid/sterol-rich domains so-called lipid rafts of the plasma membrane (PM) from mammalian cells, and more recently from leaves, cell cultures, and seedlings of higher plants. Here we show that lipid raft domains, defined as Triton X-100-insoluble membranes, can also be prepared from Medicago truncatula root PMs. These domains have been extensively characterized by ultrastructural studies as well as by analysis of their content in lipids and proteins. M. truncatula lipid domains are shown to be enriched in sphingolipids and Delta(7)-sterols, with spinasterol as the major compound, but also in steryl glycosides and acyl-steryl glycosides. A large number of proteins (i.e. 270) have been identified. Among them, receptor kinases and proteins related to signaling, cellular trafficking, and cell wall functioning were well represented whereas those involved in transport and metabolism were poorly represented. Evidence is also given for the presence of a complete PM redox system in the lipid rafts.

MeSH Terms
Cell Fractionation Medicago truncatula/metabolism,ultrastructure Membrane Lipids/chemistry,metabolism Membrane Microdomains/chemistry,metabolism,ultrastructure Oxidation-Reduction Plant Proteins/classification,isolation & purification,metabolism Plant Roots/metabolism,ultrastructure Proteomics Solubility Stigmasterol/analogs & derivatives,metabolism
Chemicals
Membrane Lipids Plant Proteins spinasterol Stigmasterol
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Lefebvre Benoit
Laboratoire des Interactions Plantes Micro-organismes, Unité Mixte de Recherche, Centre National de la Recherche Scientifique-Institut National de la Recherche Agronomique 2594/441, 31326 Castanet-Tolosan cedex, France.
Furt Fabienne
Hartmann Marie-Andrée
Michaelson Louise V
Carde Jean-Pierre
Sargueil-Boiron Françoise
Rossignol Michel
Napier Johnathan A
Cullimore Julie
Bessoule Jean-Jacques
Mongrand Sébastien
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.106.094102
Published
2007-05-00
Epub
2007-00-02
Pages
402-18
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC1913791
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.106.094102 PMC Full Text View journal details
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