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PMID: 1740112 Published · ppublish English Journal Article

Roles of U4 and U6 snRNAs in the assembly of splicing complexes.

The EMBO journal ·Vol. 11 ·No. 1 ·1992-01-00 ·Pages 335-43

Vankan P, McGuigan C, Mattaj IW

Abstract

A series of U4 and U6 snRNA mutants was analysed in Xenopus oocytes to determine whether they block splicing complex assembly or splicing itself. All the U4 and U6 mutants found to be inactive in splicing complementation resulted in defects in assembly of either U4/U6 snRNP or of splicing complexes. No mutants were found to separate the entry of U5 and U6 snRNAs into splicing complexes and neither of these RNAs was able to associate with the pre-mRNA in the absence of U4. In the absence of U6 snRNA, however, U4 entered a complex containing pre-mRNA as well as the U1 and U2 snRNAs. U6 nucleotides whose mutation resulted in specific blockage of the second step of splicing in Saccharomyces cerevisiae are shown not to be essential for splicing in the oocyte assay. The results are discussed in terms of the roles of U4 and U6 in the assembly and catalytic steps of the splicing process.

MeSH Terms
Animals Base Sequence Genetic Complementation Test Microinjections Molecular Sequence Data Mutagenesis Mutation Nucleic Acid Conformation Oocytes/metabolism RNA Splicing RNA, Small Nuclear/metabolism Ribonucleoproteins/metabolism Saccharomyces cerevisiae/metabolism Xenopus
Chemicals
RNA, Small Nuclear Ribonucleoproteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Vankan P
EMBL, Heidelberg, FRG.
McGuigan C
Mattaj I W
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47 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-01-00
Pages
335-43
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556454
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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