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PMID: 17530928 Published · ppublish English Journal Article

Zip4/Spo22 is required for class I CO formation but not for synapsis completion in Arabidopsis thaliana.

PLoS genetics ·Vol. 3 ·No. 5 ·2007-05-25 ·Pages e83

Chelysheva L, Gendrot G, Vezon D, Doutriaux MP, Mercier R, Grelon M

Abstract

In budding yeast meiosis, the formation of class I interference-sensitive crossovers requires the ZMM proteins. These ZMM proteins are essential in forming a mature synaptonemal complex, and a subset of these (Zip2, Zip3, and Zip4) has been proposed to compose the core of synapsis initiation complexes (SICs). Zip4/Spo22 functions with Zip2 to promote polymerization of Zip1 along chromosomes, making it a crucial SIC component. In higher eukaryotes, synapsis and recombination have often been correlated, but it is totally unknown how these two processes are linked. In this study, we present the characterization of a higher eukaryote SIC component homologue: Arabidopsis AtZIP4. We show that mutations in AtZIP4 belong to the same epistasis group as Atmsh4 and eliminate approximately 85% of crossovers (COs). Furthermore, genetic analyses on two adjacent intervals of Chromosome I established that the remaining COs in Atzip4 do not show interference. Lastly, immunolocalization studies showed that polymerization of the central element of the synaptonemal complex is not affected in Atzip4 background, even if it may proceed from fewer sites compared to wild type. These results reveal that Zip4 function in class I CO formation is conserved from budding yeast to Arabidopsis. On the other hand, and contrary to the situation in yeast, mutation in AtZIP4 does not prevent synapsis, showing that both aspects of the Zip4 function (i.e., class I CO maturation and synapsis) can be uncoupled.

MeSH Terms
Amino Acid Sequence Arabidopsis/cytology,genetics Arabidopsis Proteins/chemistry,genetics,metabolism Cation Transport Proteins/chemistry,genetics,metabolism Chromosome Pairing/genetics,physiology Chromosomes, Plant/genetics Crossing Over, Genetic DNA-Binding Proteins/metabolism Exons/genetics Molecular Sequence Data Mutant Proteins/isolation & purification Mutation/genetics Phenotype Pollen/cytology,metabolism Protein Transport
Chemicals
ASY1 protein, Arabidopsis Arabidopsis Proteins Cation Transport Proteins DNA-Binding Proteins Mutant Proteins Zip4 protein, Arabidopsis
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Chelysheva Liudmila
Institut Jean-Pierre Bourgin, INRA de Versailles, Station de Génétique et d'Amélioration des Plantes UR-254, France.
Gendrot Ghislaine
Vezon Daniel
Doutriaux Marie-Pascale
Mercier Raphaël
Grelon Mathilde
Conflict of Interest

Competing interests. The authors have declared that no competing interests exist.

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Article Info
Journal
PLoS genetics
Abbr.
PLoS Genet
ISSN
1553-7404
DOI
10.1371/journal.pgen.0030083
Published
2007-05-25
Pages
e83
Language
English
Region
United States
NLM ID
101239074
PMCID
PMC1877879
Subset
IM
Databases
GENBANK
AAH36016, EF176583
RefSeq
NP_012192, NP_915110, XP_692604
External Links
PubMed source DOI: 10.1371/journal.pgen.0030083 PMC Full Text View journal details
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