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PMID: 1762913 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes.

Nucleic acids research ·Vol. 19 ·No. 24 ·1991-12-25 ·Pages 6823-31

Versalovic J, Koeuth T, Lupski JR

Abstract

Dispersed repetitive DNA sequences have been described recently in eubacteria. To assess the distribution and evolutionary conservation of two distinct prokaryotic repetitive elements, consensus oligonucleotides were used in polymerase chain reaction [PCR] amplification and slot blot hybridization experiments with genomic DNA from diverse eubacterial species. Oligonucleotides matching Repetitive Extragenic Palindromic [REP] elements and Enterobacterial Repetitive Intergenic Consensus [ERIC] sequences were synthesized and tested as opposing PCR primers in the amplification of eubacterial genomic DNA. REP and ERIC consensus oligonucleotides produced clearly resolvable bands by agarose gel electrophoresis following PCR amplification. These band patterns provided unambiguous DNA fingerprints of different eubacterial species and strains. Both REP and ERIC probes hybridized preferentially to genomic DNA from Gram-negative enteric bacteria and related species. Widespread distribution of these repetitive DNA elements in the genomes of various microorganisms should enable rapid identification of bacterial species and strains, and be useful for the analysis of prokaryotic genomes.

MeSH Terms
Bacteria/genetics Base Sequence Binding Sites/genetics Consensus Sequence DNA Fingerprinting DNA, Bacterial/genetics Enterobacteriaceae/genetics Genome, Bacterial Molecular Sequence Data Oligodeoxyribonucleotides/genetics Polymerase Chain Reaction Repetitive Sequences, Nucleic Acid/genetics
Chemicals
DNA, Bacterial Oligodeoxyribonucleotides
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Versalovic J
Institute for Molecular Genetics, Baylor College of Medicine, Houston, TX 77030.
Koeuth T
Lupski J R
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.24.6823
Published
1991-12-25
Pages
6823-31
Language
English
Region
England
NLM ID
0411011
PMCID
PMC329316
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/19.24.6823 PMC Full Text View journal details
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