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PMID: 17630275 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Ethylene upregulates auxin biosynthesis in Arabidopsis seedlings to enhance inhibition of root cell elongation.

The Plant cell ·Vol. 19 ·No. 7 ·2007-07-00 ·Pages 2186-96

Swarup R, Perry P, Hagenbeek D, Van Der Straeten D, Beemster GT, Sandberg G, Bhalerao R, Ljung K, Bennett MJ

Abstract

Ethylene represents an important regulatory signal for root development. Genetic studies in Arabidopsis thaliana have demonstrated that ethylene inhibition of root growth involves another hormone signal, auxin. This study investigated why auxin was required by ethylene to regulate root growth. We initially observed that ethylene positively controls auxin biosynthesis in the root apex. We subsequently demonstrated that ethylene-regulated root growth is dependent on (1) the transport of auxin from the root apex via the lateral root cap and (2) auxin responses occurring in multiple elongation zone tissues. Detailed growth studies revealed that the ability of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid to inhibit root cell elongation was significantly enhanced in the presence of auxin. We conclude that by upregulating auxin biosynthesis, ethylene facilitates its ability to inhibit root cell expansion.

MeSH Terms
Arabidopsis/drug effects,genetics,metabolism Arabidopsis Proteins/genetics,metabolism Biological Transport/drug effects Ethylenes/pharmacology Gene Expression Regulation, Plant/drug effects Genes, Plant Glucuronidase/metabolism Indoleacetic Acids/metabolism Models, Biological Plant Roots/cytology,drug effects,growth & development Seedlings/drug effects,metabolism Up-Regulation/drug effects
Chemicals
AUX1 protein, Arabidopsis Arabidopsis Proteins Ethylenes Indoleacetic Acids ethylene Glucuronidase
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Swarup Ranjan
School of Biosciences and Centre for Plant Integrative Biology, University of Nottingham, LE12 5RD Nottingham, United Kingdom.
Perry Paula
Hagenbeek Dik
Van Der Straeten Dominique
Beemster Gerrit T S
Sandberg Göran
Bhalerao Rishikesh
Ljung Karin
Bennett Malcolm J
References (40)
40 references, click to expand
  1. The axr4 auxin-resistant mutants of Arabidopsis thaliana define a gene important for root gravitropism and lateral root initiation.
    Plant J. 1995 Feb;7(2):211-20 PMID: 7704045
  2. Multilevel interactions between ethylene and auxin in Arabidopsis roots.
    Plant Cell. 2007 Jul;19(7):2169-85 PMID: 17630276
  3. Five components of the ethylene-response pathway identified in a screen for weak ethylene-insensitive mutants in Arabidopsis.
    Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2992-7 PMID: 12606727
  4. Regulation of cell length in the Arabidopsis thaliana root by the ethylene precursor 1-aminocyclopropane- 1-carboxylic acid: a matter of apoplastic reactions.
    New Phytol. 2005 Dec;168(3):541-50 PMID: 16313637
  5. Transcriptional profiling by cDNA-AFLP and microarray analysis reveals novel insights into the early response to ethylene in Arabidopsis.
    Plant J. 2004 Aug;39(4):537-59 PMID: 15272873
  6. Hormones act downstream of TTG and GL2 to promote root hair outgrowth during epidermis development in the Arabidopsis root.
    Plant Cell. 1996 Sep;8(9):1505-17 PMID: 8837505
  7. Sites and regulation of auxin biosynthesis in Arabidopsis roots.
    Plant Cell. 2005 Apr;17(4):1090-104 PMID: 15772288
  8. Genetic analysis of ethylene signal transduction in Arabidopsis thaliana: five novel mutant loci integrated into a stress response pathway.
    Genetics. 1995 Mar;139(3):1393-409 PMID: 7768447
  9. A Link between ethylene and auxin uncovered by the characterization of two root-specific ethylene-insensitive mutants in Arabidopsis.
    Plant Cell. 2005 Aug;17(8):2230-42 PMID: 15980261
  10. Structure-function analysis of the presumptive Arabidopsis auxin permease AUX1.
    Plant Cell. 2004 Nov;16(11):3069-83 PMID: 15486104
  11. Soil compaction. A role for ethylene in regulating leaf expansion and shoot growth in tomato?
    Plant Physiol. 1999 Dec;121(4):1227-38 PMID: 10594109
  12. The AXR1 and AUX1 genes of Arabidopsis function in separate auxin-response pathways.
    Plant J. 1995 Oct;8(4):561-9 PMID: 11536712
  13. Auxin is a positive regulator for ethylene-mediated response in the growth of Arabidopsis roots.
    Plant Cell Physiol. 2001 Mar;42(3):301-7 PMID: 11266581
  14. The POLARIS peptide of Arabidopsis regulates auxin transport and root growth via effects on ethylene signaling.
    Plant Cell. 2006 Nov;18(11):3058-72 PMID: 17138700
  15. Auxin cross-talk: integration of signalling pathways to control plant development.
    Plant Mol Biol. 2002 Jun-Jul;49(3-4):411-26 PMID: 12036264
  16. A new algorithm for computational image analysis of deformable motion at high spatial and temporal resolution applied to root growth. Roughly uniform elongation in the meristem and also, after an abrupt acceleration, in the elongation zone.
    Plant Physiol. 2003 Jul;132(3):1138-48 PMID: 12857796
  17. Arabidopsis ethylene-response gene ETR1: similarity of product to two-component regulators.
    Science. 1993 Oct 22;262(5133):539-44 PMID: 8211181
  18. Stunted plant 1 mediates effects of cytokinin, but not of auxin, on cell division and expansion in the root of Arabidopsis.
    Plant Physiol. 2000 Dec;124(4):1718-27 PMID: 11115888
  19. AXR4 is required for localization of the auxin influx facilitator AUX1.
    Science. 2006 May 26;312(5777):1218-20 PMID: 16690816
  20. A dominant mutation in Arabidopsis confers resistance to auxin, ethylene and abscisic acid.
    Mol Gen Genet. 1990 Jul;222(2-3):377-83 PMID: 2148800
  21. Nuclear events in ethylene signaling: a transcriptional cascade mediated by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSE-FACTOR1.
    Genes Dev. 1998 Dec 1;12(23):3703-14 PMID: 9851977
  22. A new mathematical model for relative quantification in real-time RT-PCR.
    Nucleic Acids Res. 2001 May 1;29(9):e45 PMID: 11328886
  23. Organization and cell differentiation in lateral roots of Arabidopsis thaliana.
    Development. 1997 Jan;124(1):33-44 PMID: 9006065
  24. The ethylene signaling pathway.
    Science. 2004 Nov 26;306(5701):1513-5 PMID: 15567852
  25. In the early response of Arabidopsis roots to ethylene, cell elongation is up- and down-regulated and uncoupled from differentiation.
    Plant Physiol. 2001 Feb;125(2):519-22 PMID: 11161008
  26. Root formation in ethylene-insensitive plants.
    Plant Physiol. 1999 Sep;121(1):53-60 PMID: 10482660
  27. Association of the Arabidopsis CTR1 Raf-like kinase with the ETR1 and ERS ethylene receptors.
    Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):5401-6 PMID: 9560288
  28. Analysis of cell division and elongation underlying the developmental acceleration of root growth in Arabidopsis thaliana.
    Plant Physiol. 1998 Apr;116(4):1515-26 PMID: 9536070
  29. Polar PIN localization directs auxin flow in plants.
    Science. 2006 May 12;312(5775):883 PMID: 16601151
  30. High-affinity auxin transport by the AUX1 influx carrier protein.
    Curr Biol. 2006 Jun 6;16(11):1123-7 PMID: 16677815
  31. Ethylene is a positive regulator of root hair development in Arabidopsis thaliana.
    Plant J. 1995 Dec;8(6):943-8 PMID: 8580964
  32. Root gravitropism requires lateral root cap and epidermal cells for transport and response to a mobile auxin signal.
    Nat Cell Biol. 2005 Nov;7(11):1057-65 PMID: 16244669
  33. CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encodes a member of the raf family of protein kinases.
    Cell. 1993 Feb 12;72(3):427-41 PMID: 8431946
  34. Localization of the auxin permease AUX1 suggests two functionally distinct hormone transport pathways operate in the Arabidopsis root apex.
    Genes Dev. 2001 Oct 15;15(20):2648-53 PMID: 11641271
  35. Formation of Di-Isodityrosine and Loss of Isodityrosine in the Cell Walls of Tomato Cell-Suspension Cultures Treated with Fungal Elicitors or H2O2.
    Plant Physiol. 1997 Sep;115(1):87-92 PMID: 12223793
  36. Mutations in the AXR3 gene of Arabidopsis result in altered auxin response including ectopic expression from the SAUR-AC1 promoter.
    Plant J. 1996 Sep;10(3):403-13 PMID: 8811856
  37. EIR1, a root-specific protein involved in auxin transport, is required for gravitropism in Arabidopsis thaliana.
    Genes Dev. 1998 Jul 15;12(14):2175-87 PMID: 9679062
  38. Ethylene regulates root growth through effects on auxin biosynthesis and transport-dependent auxin distribution.
    Plant Cell. 2007 Jul;19(7):2197-212 PMID: 17630274
  39. The aux1 Mutation of Arabidopsis Confers Both Auxin and Ethylene Resistance.
    Plant Physiol. 1990 Nov;94(3):1462-6 PMID: 16667854
  40. A polymorphic bipartite motif signals nuclear targeting of early auxin-inducible proteins related to PS-IAA4 from pea (Pisum sativum).
    Plant J. 1995 Jul;8(1):87-96 PMID: 7655509
Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.107.052100
Published
2007-07-00
Epub
2007-00-13
Pages
2186-96
Language
English
Region
England
NLM ID
9208688
PMCID
PMC1955695
Subset
IM
Grants
Biotechnology and Biological Sciences Research Council · BB/D019613/1 · United Kingdom
External Links
PubMed source DOI: 10.1105/tpc.107.052100 PMC Full Text View journal details
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