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PMID: 1766432 Published · ppublish English Journal Article

The gene for a halophilic glutamate dehydrogenase: sequence, transcription analysis and phylogenetic implications.

Molecular & general genetics : MGG ·Vol. 230 ·No. 3 ·1991-12-00 ·Pages 345-52

Benachenhou N, Baldacci G

Abstract

We have isolated and sequenced the gene for a putative NADP-dependent glutamate dehydrogenase from the extremely halophilic archaebacterium Halobacterium salinarium. This gene is transcribed as a unique RNA molecule of about 1700 nucleotides. The 5' end of the transcript contains characteristic consensus transcription initiation and promoter sequences observed in halophilic archaebacteria. The encoded polypeptide, with a predicted length of 435 amino acids, shows significant overall homology and conservation of functional domains when compared with different eubacterial and eukaryotic glutamate dehydrogenases. Surprisingly, the archaebacterial protein shares a larger number of identical amino acid residues with homologous polypeptides from higher eukaryotes than with those from unicellular eukaryotes and eubacteria.

Related Genes
gdhA
MeSH Terms
Amino Acid Sequence Amino Acids/analysis Base Sequence Codon/genetics Glutamate Dehydrogenase/chemistry,genetics,metabolism Halobacterium/enzymology,genetics Molecular Sequence Data NADP/metabolism Phylogeny Protein Conformation Transcription, Genetic
Chemicals
Amino Acids Codon NADP Glutamate Dehydrogenase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Benachenhou N
Institut de Génétique et Microbiologie, Université Paris-Sud, Orsay, France.
Baldacci G
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00280290
Published
1991-12-00
Pages
345-52
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
S55527, S55528, S55529, S55530, S55531, S55532, S63909, S69885, S75579, X63837
External Links
PubMed source DOI: 10.1007/BF00280290
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