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PMID: 17665215 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Structure of two melon regions reveals high microsynteny with sequenced plant species.

Molecular genetics and genomics : MGG ·Vol. 278 ·No. 6 ·2007-12-00 ·Pages 611-22

Deleu W, González V, Monfort A, Bendahmane A, Puigdomènech P, Arús P, Garcia-Mas J

Abstract

In this study, two melon bacterial artificial chromosome (BAC) clones have been sequenced and annotated. BAC 1-21-10 spans 92 kb and contains the nsv locus conferring resistance to the Melon Necrotic Spot Virus (MNSV) in melon linkage group 11. BAC 13J4 spans 98 kb and belongs to a BAC contig containing resistance gene homologues, extending a previous sequenced region of 117 kb in linkage group 4. Both regions have microsyntenic relationships to the model plant species Arabidopsis thaliana, and to Medicago truncatula and Populus trichocarpa. The network of synteny found between melon and each of the sequenced genomes reflects the polyploid structure of Arabidopsis, Populus, and Medicago genomes due to whole genome duplications (WGD). A detailed analysis revealed that both melon regions have a lower relative syntenic quality with Arabidopsis (eurosid II) than when compared to Populus and Medicago (eurosid I). Although phylogenetically Cucurbitales seem to be closer to Fabales than to Malphigiales, synteny was higher between both melon regions and Populus. Presented data imply that the recently completed Populus genome sequence could preferentially be used to obtain positional information in melon, based on microsynteny.

MeSH Terms
Chromosome Mapping Chromosomes, Artificial, Bacterial Chromosomes, Plant Cucurbitaceae/genetics Immunity, Innate/genetics Molecular Sequence Data Repetitive Sequences, Nucleic Acid Sequence Analysis, DNA Synteny
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Deleu Wim
IRTA, Centre de Recerca en Agrigenòmica CSIC-IRTA-UAB, Carretera de Cabrils Km2, 08348 Cabrils, Barcelona, Spain.
González Víctor
Monfort Amparo
Bendahmane Abdelhafid
Puigdomènech Pere
Arús Pere
Garcia-Mas Jordi
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Article Info
Journal
Molecular genetics and genomics : MGG
Abbr.
Mol Genet Genomics
ISSN
1617-4615
DOI
10.1007/s00438-007-0277-2
Published
2007-12-00
Epub
2007-00-31
Pages
611-22
Language
English
Region
Germany
NLM ID
101093320
Subset
IM
Databases
GENBANK
EF188258, EF657230
External Links
PubMed source DOI: 10.1007/s00438-007-0277-2 View journal details
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