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PMID: 17671168 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

CDK-5 regulates the abundance of GLR-1 glutamate receptors in the ventral cord of Caenorhabditis elegans.

Molecular biology of the cell ·Vol. 18 ·No. 10 ·2007-10-00 ·Pages 3883-93

Juo P, Harbaugh T, Garriga G, Kaplan JM

Abstract

The proline-directed kinase Cdk5 plays a role in several aspects of neuronal development. Here, we show that CDK-5 activity regulates the abundance of the glutamate receptor GLR-1 in the ventral cord of Caenorhabditis elegans and that it produces corresponding changes in GLR-1-dependent behaviors. Loss of CDK-5 activity results in decreased abundance of GLR-1 in the ventral cord, accompanied by accumulation of GLR-1 in neuronal cell bodies. Genetic analysis of cdk-5 and the clathrin adaptin unc-11 AP180 suggests that CDK-5 functions prior to endocytosis at the synapse. The scaffolding protein LIN-10/Mint-1 also regulates GLR-1 abundance in the nerve cord. CDK-5 phosphorylates LIN-10/Mint-1 in vitro and bidirectionally regulates the abundance of LIN-10/Mint-1 in the ventral cord. We propose that CDK-5 promotes the anterograde trafficking of GLR-1 and that phosphorylation of LIN-10 may play a role in this process.

MeSH Terms
Animals Caenorhabditis elegans/enzymology Caenorhabditis elegans Proteins/metabolism Cyclin-Dependent Kinase 5/chemistry,metabolism Down-Regulation/genetics Endocytosis Membrane Proteins/metabolism Mutation/genetics Nervous System/enzymology Phosphorylation Protein Structure, Tertiary Protein Subunits/metabolism R-SNARE Proteins/metabolism Receptors, AMPA/metabolism Recombinant Fusion Proteins/metabolism
Chemicals
Caenorhabditis elegans Proteins Membrane Proteins Protein Subunits R-SNARE Proteins Receptors, AMPA Recombinant Fusion Proteins glr-1 protein, C elegans lin-10 protein, C elegans Cyclin-Dependent Kinase 5
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Juo Peter
Department of Molecular Biology, Massachusetts General Hospital, and Department of Genetics, Harvard Medical School, Boston, MA 02114, USA. [email protected]
Harbaugh Tom
Garriga Gian
Kaplan Joshua M
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e06-09-0818
Email
[email protected]
Published
2007-10-00
Epub
2007-00-01
Pages
3883-93
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC1995742
Subset
IM
Grants
NINDS NIH HHS · R01 NS059953-01 · United States
NINDS NIH HHS · NS59953 · United States
NINDS NIH HHS · R01 NS032196 · United States
NINDS NIH HHS · F32NS10957 · United States
NINDS NIH HHS · R56 NS059953 · United States
NINDS NIH HHS · F32 NS010957 · United States
NINDS NIH HHS · R01 NS059953 · United States
NINDS NIH HHS · NS32196 · United States
NINDS NIH HHS · R37 NS032196 · United States
External Links
PubMed source DOI: 10.1091/mbc.e06-09-0818 PMC Full Text View journal details
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