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PMID: 17827294 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

DivL performs critical cell cycle functions in Caulobacter crescentus independent of kinase activity.

Journal of bacteriology ·Vol. 189 ·No. 22 ·2007-11-00 ·Pages 8308-20

Reisinger SJ, Huntwork S, Viollier PH, Ryan KR

Abstract

The Caulobacter cell cycle is regulated by a network of two-component signal transduction proteins. Phosphorylation and stability of the master transcriptional regulator CtrA are controlled by the CckA-ChpT phosphorelay, and CckA activity is modulated by another response regulator, DivK. In a screen to identify suppressors of the cold-sensitive divK341 mutant, we found point mutations in the essential gene divL. DivL is similar to histidine kinases but has a tyrosine instead of a histidine at the conserved phosphorylation site (Y550). Surprisingly, we found that the ATPase domain of DivL is not essential for Caulobacter viability. We show that DivL selectively affects CtrA phosphorylation but not CtrA proteolysis, indicating that DivL acts in a pathway independent of the CckA-ChpT phosphorelay. divL can be deleted in a strain overproducing the phosphomimetic protein CtrAD51E, but unlike DeltactrA cells expressing CtrAD51E, this strain is profoundly impaired in the control of chromosome replication and cell division. Thus, DivL performs a second function in addition to promoting CtrA phosphorylation. DivL is required for bipolar DivK localization and positively regulates DivK phosphorylation. Our results show that DivL controls two key cell cycle regulators, CtrA and DivK, and that phosphoryl transfer is not DivL's essential cellular activity.

MeSH Terms
Alleles Bacterial Proteins/genetics,metabolism Caulobacter crescentus/cytology,enzymology,genetics,metabolism Cell Cycle/physiology DNA-Binding Proteins/metabolism Gene Expression Regulation, Bacterial Histidine Kinase Mutation Phosphorylation Protein Kinases/genetics,metabolism Protein Transport Transcription Factors/metabolism
Chemicals
Bacterial Proteins CtrA protein, Caulobacter DNA-Binding Proteins DivK protein, Caulobacter crescentus Transcription Factors Protein Kinases Histidine Kinase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Reisinger Sarah J
Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, California 94720, USA.
Huntwork Sarah
Viollier Patrick H
Ryan Kathleen R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
1098-5530
DOI
10.1128/JB.00868-07
Published
2007-11-00
Epub
2007-00-07
Pages
8308-20
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC2168681
Subset
IM
Grants
NIGMS NIH HHS · R01 GM032506 · United States
NIGMS NIH HHS · R37 GM032506 · United States
NIGMS NIH HHS · GM032506 · United States
External Links
PubMed source DOI: 10.1128/JB.00868-07 PMC Full Text View journal details
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