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PMID: 17918201 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

TLR-mediated stimulation of APC: Distinct cytokine responses of B cells and dendritic cells.

European journal of immunology ·Vol. 37 ·No. 11 ·2007-11-00 ·Pages 3040-53

Barr TA, Brown S, Ryan G, Zhao J, Gray D

Abstract

In addition to their role in humoral immunity, B lymphocytes are important antigen-presenting cells (APC). In the same way as other APC, B cells make cytokines upon activation and have the potential to modulate T cell responses. In this study, we investigated which mouse B cell subsets are the most potent cytokine producers, and examined the role of Toll-like receptors (TLR) in the control of secretion of IL-6, IL-10, IL-12 and IFN-gamma by B cells. Production of some cytokines was restricted to particular subsets. Marginal zone and B1 cells were the predominant source of B cell IL-10 in the spleen. Conversely, follicular B cells were found to express IFN-gamma mRNA directly ex vivo. The nature of the activating stimulus dramatically influenced the cytokine made by B cells. Thus, in response to combined TLR stimulation, or via phorbol esters, IFN-gamma was secreted. IL-10 was elicited by T-dependent activation or stimulation through TLR2, 4 or 9. This pattern of cytokine expression contrasts with that elicited from dendritic cells. QRT-PCR array data indicate that this may be due to differential expression of TLR signalling molecules, effectors and adaptors. Our data highlight the potentially unique nature of immune modulation when B cells act as APC.

MeSH Terms
Animals B-Lymphocyte Subsets B-Lymphocytes/immunology,metabolism Cytokines/metabolism DNA Primers Dendritic Cells/immunology,metabolism Enzyme-Linked Immunosorbent Assay Flow Cytometry Gene Expression Lymphocyte Activation/immunology Mice RNA, Messenger/analysis Reverse Transcriptase Polymerase Chain Reaction Toll-Like Receptors/immunology,metabolism
Chemicals
Cytokines DNA Primers RNA, Messenger Toll-Like Receptors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Barr Tom A
Institute of Immunology and Infection Research, School of Biological Science, University of Edinburgh, Edinburgh, UK. [email protected]
Brown Sheila
Ryan Gemma
Zhao Jiexin
Gray David
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Article Info
Journal
European journal of immunology
Abbr.
Eur J Immunol
ISSN
0014-2980
DOI
10.1002/eji.200636483
Email
[email protected]
Published
2007-11-00
Pages
3040-53
Language
English
Region
Germany
NLM ID
1273201
PMCID
PMC2699383
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1002/eji.200636483 PMC Full Text View journal details
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