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PMID: 17923688 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Requirement of Nse1, a subunit of the Smc5-Smc6 complex, for Rad52-dependent postreplication repair of UV-damaged DNA in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 27 ·No. 23 ·2007-12-00 ·Pages 8409-18

Santa Maria SR, Gangavarapu V, Johnson RE, Prakash L, Prakash S

Abstract

In Saccharomyces cerevisiae, postreplication repair (PRR) of UV-damaged DNA occurs by a Rad6-Rad18- and an Mms2-Ubc13-Rad5-dependent pathway or by a Rad52-dependent pathway. The Rad5 DNA helicase activity is specialized for promoting replication fork regression and template switching; previously, we suggested a role for the Rad5-dependent PRR pathway when the lesion is located on the leading strand and a role for the Rad52 pathway when the lesion is located on the lagging strand. In this study, we present evidence for the requirement of Nse1, a subunit of the Smc5-Smc6 complex, in Rad52-dependent PRR, and our genetic analyses suggest a role for the Nse1 and Mms21 E3 ligase activities associated with this complex in this repair mode. We discuss the possible ways by which the Smc5-Smc6 complex, including its associated ubiquitin ligase and SUMO ligase activities, might contribute to the Rad52-dependent nonrecombinational and recombinational modes of PRR.

MeSH Terms
Amino Acid Motifs Amino Acid Sequence Cell Cycle Proteins/metabolism DNA Damage DNA Repair/radiation effects DNA Replication/radiation effects DNA, Fungal/metabolism Epistasis, Genetic Molecular Sequence Data Mutant Proteins/isolation & purification,metabolism Mutation/genetics Nuclear Proteins/chemistry,metabolism Proliferating Cell Nuclear Antigen/metabolism Protein Subunits/metabolism Rad52 DNA Repair and Recombination Protein/metabolism SUMO-1 Protein/metabolism Saccharomyces cerevisiae/cytology,enzymology,radiation effects Saccharomyces cerevisiae Proteins/chemistry,metabolism Ubiquitin-Protein Ligases/metabolism Ultraviolet Rays
Chemicals
Cell Cycle Proteins DNA, Fungal Mms21 protein, S cerevisiae Mutant Proteins NSE1 protein, S cerevisiae Nuclear Proteins Proliferating Cell Nuclear Antigen Protein Subunits RAD52 protein, S cerevisiae Rad52 DNA Repair and Recombination Protein SMC5 protein, S cerevisiae SMC6 protein, S cerevisiae SUMO-1 Protein Saccharomyces cerevisiae Proteins Ubiquitin-Protein Ligases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Santa Maria Sergio R
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch at Galveston, 301 University Blvd., Galveston, TX 77555-1061, USA.
Gangavarapu Venkateswarlu
Johnson Robert E
Prakash Louise
Prakash Satya
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
1098-5549
DOI
10.1128/MCB.01543-07
Published
2007-12-00
Epub
2007-00-08
Pages
8409-18
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC2169175
Subset
IM
Grants
NCI NIH HHS · R01 CA107650 · United States
NCI NIH HHS · CA107650 · United States
External Links
PubMed source DOI: 10.1128/MCB.01543-07 PMC Full Text View journal details
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