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PMID: 17984324 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Function and dynamics of PKD2 in Chlamydomonas reinhardtii flagella.

The Journal of cell biology ·Vol. 179 ·No. 3 ·2007-11-05 ·Pages 501-14

Huang K, Diener DR, Mitchell A, Pazour GJ, Witman GB, Rosenbaum JL

Abstract

To analyze the function of ciliary polycystic kidney disease 2 (PKD2) and its relationship to intraflagellar transport (IFT), we cloned the gene encoding Chlamydomonas reinhardtii PKD2 (CrPKD2), a protein with the characteristics of PKD2 family members. Three forms of this protein (210, 120, and 90 kD) were detected in whole cells; the two smaller forms are cleavage products of the 210-kD protein and were the predominant forms in flagella. In cells expressing CrPKD2-GFP, about 10% of flagellar CrPKD2-GFP was observed moving in the flagellar membrane. When IFT was blocked, fluorescence recovery after photobleaching of flagellar CrPKD2-GFP was attenuated and CrPKD2 accumulated in the flagella. Flagellar CrPKD2 increased fourfold during gametogenesis, and several CrPKD2 RNA interference strains showed defects in flagella-dependent mating. These results suggest that the CrPKD2 cation channel is involved in coupling flagellar adhesion at the beginning of mating to the increase in flagellar calcium required for subsequent steps in mating.

MeSH Terms
Amino Acid Sequence Animals Biological Transport Cell Adhesion Chlamydomonas reinhardtii/metabolism Cloning, Molecular Flagella/metabolism Green Fluorescent Proteins/metabolism Models, Biological Molecular Sequence Data Nucleic Acid Hybridization Protein Structure, Tertiary RNA Interference TRPP Cation Channels/chemistry,metabolism Temperature
Chemicals
TRPP Cation Channels polycystic kidney disease 2 protein Green Fluorescent Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Huang Kaiyao
Department of Molecular Cell and Developmental Biology, Yale University, New Haven, CT 06520, USA.
Diener Dennis R
Mitchell Aaron
Pazour Gregory J
Witman George B
Rosenbaum Joel L
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200704069
Published
2007-11-05
Pages
501-14
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2064795
Subset
IM
Grants
NIGMS NIH HHS · GM060992 · United States
NIGMS NIH HHS · GM30626 · United States
NIGMS NIH HHS · R01 GM030626 · United States
NIGMS NIH HHS · R37 GM030626 · United States
NIGMS NIH HHS · R01 GM014642 · United States
NIGMS NIH HHS · R37 GM014642 · United States
NIGMS NIH HHS · R01 GM060992 · United States
NIGMS NIH HHS · GM014642 · United States
External Links
PubMed source DOI: 10.1083/jcb.200704069 PMC Full Text View journal details
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