Home LiteratureArticle Details
PMID: 18272786 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Pxl1p, a paxillin-related protein, stabilizes the actomyosin ring during cytokinesis in fission yeast.

Molecular biology of the cell ·Vol. 19 ·No. 4 ·2008-04-00 ·Pages 1680-92

Ge W, Balasubramanian MK

Abstract

Paxillins are a family of conserved LIM domain-containing proteins that play important roles in the function and integrity of the actin cytoskeleton. Although paxillins have been extensively characterized by cell biological and biochemical approaches, genetic studies are relatively scarce. Here, we identify and characterize a paxillin-related protein Pxl1p in the fission yeast Schizosaccharomyces pombe. Pxl1p is a component of the fission yeast actomyosin ring, a structure that is essential for cytokinesis. Cells deleted for pxl1 display a novel phenotype characterized by a splitting of the actomyosin ring in late anaphase, leading to the formation of two rings of which only one undergoes constriction. In addition, the rate of actomyosin ring constriction is slower in the absence of Pxl1p. pxl1Delta mutants display strong genetic interactions with mutants defective in IQGAP-related protein Rng2p and mutants defective in components of the fission yeast type II myosin machinery. Collectively, these results suggest that Pxl1p might cooperate with type II myosin and Rng2p-IQGAP to regulate actomyosin ring constriction as well as to maintain its integrity during constriction.

MeSH Terms
Actomyosin/chemistry,genetics,metabolism Amino Acid Sequence Base Sequence Cell Cycle Proteins/chemistry,genetics,metabolism Cytokinesis/genetics,physiology Cytoskeletal Proteins/chemistry,genetics,metabolism DNA Primers/genetics DNA, Fungal/genetics GTPase-Activating Proteins/chemistry,genetics,metabolism Gene Deletion Genes, Fungal Molecular Sequence Data Mutation Myosin Type II/chemistry,genetics,metabolism Paxillin/chemistry,genetics,metabolism Phenotype Recombinant Fusion Proteins/chemistry,genetics,metabolism Schizosaccharomyces/cytology,genetics,metabolism Schizosaccharomyces pombe Proteins/chemistry,genetics,metabolism Sequence Homology, Amino Acid ras GTPase-Activating Proteins/chemistry,genetics,metabolism
Chemicals
Cell Cycle Proteins Cytoskeletal Proteins DNA Primers DNA, Fungal GTPase-Activating Proteins IQ motif containing GTPase activating protein 1 Paxillin Pxl1 protein, S pombe Recombinant Fusion Proteins Rng2 protein, S pombe Schizosaccharomyces pombe Proteins ras GTPase-Activating Proteins Actomyosin Myosin Type II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ge Wanzhong
Cell Division Laboratory, Temasek Life Sciences Laboratory and the Department of Biological Sciences, National University of Singapore, Singapore.
Balasubramanian Mohan K
References (54)
54 references, click to expand
  1. Molecular genetic analysis of fission yeast Schizosaccharomyces pombe.
    Methods Enzymol. 1991;194:795-823 PMID: 2005825
  2. The novel fission yeast protein Pal1p interacts with Hip1-related Sla2p/End4p and is involved in cellular morphogenesis.
    Mol Biol Cell. 2005 Sep;16(9):4124-38 PMID: 15975911
  3. Split decisions: coordinating cytokinesis in yeast.
    Trends Cell Biol. 2005 Jan;15(1):10-8 PMID: 15653073
  4. Fission yeast Clp1p phosphatase regulates G2/M transition and coordination of cytokinesis with cell cycle progression.
    Curr Biol. 2001 Jun 26;11(12):931-40 PMID: 11448769
  5. Imaging green fluorescent protein fusions in living fission yeast cells.
    Methods. 2004 Jul;33(3):220-5 PMID: 15157889
  6. Novel cysteine-rich motif and homeodomain in the product of the Caenorhabditis elegans cell lineage gene lin-11.
    Nature. 1990 Apr 26;344(6269):876-9 PMID: 1970421
  7. Growth polarity and cytokinesis in fission yeast: the role of the cytoskeleton.
    J Cell Sci Suppl. 1986;5:229-41 PMID: 3477553
  8. Cytokinesis in fission yeast Schizosaccharomyces pombe.
    Methods Enzymol. 1997;283:494-506 PMID: 9251043
  9. Splitting of the fission yeast septum.
    FEMS Yeast Res. 2007 Sep;7(6):761-70 PMID: 17596184
  10. The small GTPase Rho3 and the diaphanous/formin For3 function in polarized cell growth in fission yeast.
    J Cell Sci. 2002 Dec 1;115(Pt 23):4629-39 PMID: 12415007
  11. Isolation and characterization of fission yeast mutants defective in the assembly and placement of the contractile actin ring.
    J Cell Sci. 1996 Jan;109 ( Pt 1):131-42 PMID: 8834798
  12. The Schizosaccharomyces pombe actin-related protein, Arp3, is a component of the cortical actin cytoskeleton and interacts with profilin.
    EMBO J. 1996 Dec 2;15(23):6438-46 PMID: 8978671
  13. Pxl1p, a paxillin-like protein in Saccharomyces cerevisiae, may coordinate Cdc42p and Rho1p functions during polarized growth.
    Mol Biol Cell. 2004 Sep;15(9):3977-85 PMID: 15215315
  14. Identification of Myo3, a second type-II myosin heavy chain in the fission yeast Schizosaccharomyces pombe.
    FEBS Lett. 1997 Dec 29;420(2-3):161-6 PMID: 9459302
  15. Yeasts make their mark.
    Nat Cell Biol. 2003 Apr;5(4):294-9 PMID: 12669083
  16. Asymmetric segregation on spindle poles of the Schizosaccharomyces pombe septum-inducing protein kinase Cdc7p.
    Genes Dev. 1998 Jan 1;12(1):84-94 PMID: 9420333
  17. Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe.
    Yeast. 1998 Jul;14(10):943-51 PMID: 9717240
  18. The PXL1 gene of Saccharomyces cerevisiae encodes a paxillin-like protein functioning in polarized cell growth.
    Mol Biol Cell. 2004 Apr;15(4):1904-17 PMID: 14767053
  19. Roles of the fission yeast formin for3p in cell polarity, actin cable formation and symmetric cell division.
    Curr Biol. 2001 Oct 30;11(21):1656-65 PMID: 11696322
  20. The LIM motif defines a specific zinc-binding protein domain.
    Proc Natl Acad Sci U S A. 1993 May 15;90(10):4404-8 PMID: 8506279
  21. Identification of cell cycle-regulated genes in fission yeast.
    Mol Biol Cell. 2005 Mar;16(3):1026-42 PMID: 15616197
  22. Events at the end of mitosis in the budding and fission yeasts.
    J Cell Sci. 2003 Nov 1;116(Pt 21):4263-75 PMID: 14514882
  23. Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast.
    J Cell Biol. 2006 Jul 31;174(3):391-402 PMID: 16864655
  24. Flp1, a fission yeast orthologue of the s. cerevisiae CDC14 gene, is not required for cyclin degradation or rum1p stabilisation at the end of mitosis.
    J Cell Sci. 2001 Jul;114(Pt 14):2649-64 PMID: 11683392
  25. High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe.
    Nucleic Acids Res. 1990 Nov 25;18(22):6485-9 PMID: 2251111
  26. The dmf1/mid1 gene is essential for correct positioning of the division septum in fission yeast.
    Genes Dev. 1996 Nov 1;10(21):2707-19 PMID: 8946912
  27. SIN and the art of splitting the fission yeast cell.
    Curr Biol. 2004 Sep 7;14(17):R722-30 PMID: 15341766
  28. Sid4p is required to localize components of the septation initiation pathway to the spindle pole body in fission yeast.
    Proc Natl Acad Sci U S A. 2000 May 9;97(10):5249-54 PMID: 10805785
  29. A transcriptional pathway for cell separation in fission yeast.
    Cell Cycle. 2005 Jan;4(1):39-41 PMID: 15611619
  30. The alpha-glucanase Agn1p is required for cell separation in Schizosaccharomyces pombe.
    Biol Cell. 2005 Jul;97(7):569-76 PMID: 15850449
  31. The cell cycle-regulated genes of Schizosaccharomyces pombe.
    PLoS Biol. 2005 Jul;3(7):e225 PMID: 15966770
  32. Myosin-II reorganization during mitosis is controlled temporally by its dephosphorylation and spatially by Mid1 in fission yeast.
    J Cell Biol. 2004 Jun 7;165(5):685-95 PMID: 15184401
  33. Genetic control of fission yeast cell wall synthesis: the genes involved in wall biogenesis and their interactions in Schizosaccharomyces pombe.
    Genes Genet Syst. 1998 Aug;73(4):181-91 PMID: 9880916
  34. Type II myosin regulatory light chain relieves auto-inhibition of myosin-heavy-chain function.
    Nat Cell Biol. 2000 Nov;2(11):855-8 PMID: 11056543
  35. Analysis of mid1p, a protein required for placement of the cell division site, reveals a link between the nucleus and the cell surface in fission yeast.
    Mol Biol Cell. 2000 Aug;11(8):2757-73 PMID: 10930468
  36. Paxillin is required for cell-substrate adhesion, cell sorting and slug migration during Dictyostelium development.
    J Cell Sci. 2005 Sep 15;118(Pt 18):4295-310 PMID: 16155255
  37. Periodic gene expression program of the fission yeast cell cycle.
    Nat Genet. 2004 Aug;36(8):809-17 PMID: 15195092
  38. Mid2p stabilizes septin rings during cytokinesis in fission yeast.
    J Cell Biol. 2003 Mar 31;160(7):1083-92 PMID: 12654901
  39. Role of the alpha-glucanase Agn1p in fission-yeast cell separation.
    Mol Biol Cell. 2004 Aug;15(8):3903-14 PMID: 15194814
  40. Schizosaccharomyces pombe cdc4+ gene encodes a novel EF-hand protein essential for cytokinesis.
    J Cell Biol. 1995 Aug;130(3):651-60 PMID: 7622565
  41. The multiprotein exocyst complex is essential for cell separation in Schizosaccharomyces pombe.
    Mol Biol Cell. 2002 Feb;13(2):515-29 PMID: 11854409
  42. Paxillin: adapting to change.
    Physiol Rev. 2004 Oct;84(4):1315-39 PMID: 15383653
  43. End4/Sla2 is involved in establishment of a new growth zone in Schizosaccharomyces pombe.
    J Cell Sci. 2005 May 1;118(Pt 9):1843-50 PMID: 15827087
  44. Requirements of fission yeast septins for complex formation, localization, and function.
    Mol Biol Cell. 2004 Dec;15(12):5551-64 PMID: 15385632
  45. The endo-beta-1,3-glucanase eng1p is required for dissolution of the primary septum during cell separation in Schizosaccharomyces pombe.
    J Cell Sci. 2003 May 1;116(Pt 9):1689-98 PMID: 12665550
  46. The S. pombe rlc1 gene encodes a putative myosin regulatory light chain that binds the type II myosins myo3p and myo2p.
    J Cell Sci. 2000 Dec;113 Pt 23:4157-63 PMID: 11069761
  47. Cdc42p GTPase is involved in controlling polarized cell growth in Schizosaccharomyces pombe.
    Mol Cell Biol. 1994 Feb;14(2):1075-83 PMID: 8289788
  48. Identification of a second myosin-II in Schizosaccharomyces pombe: Myp2p is conditionally required for cytokinesis.
    Mol Biol Cell. 1997 Dec;8(12):2693-705 PMID: 9398685
  49. Role of bud6p and tea1p in the interaction between actin and microtubules for the establishment of cell polarity in fission yeast.
    Curr Biol. 2001 Jun 5;11(11):836-45 PMID: 11516644
  50. The Clp1p/Flp1p phosphatase ensures completion of cytokinesis in response to minor perturbation of the cell division machinery in Schizosaccharomyces pombe.
    J Cell Sci. 2004 Aug 1;117(Pt 17):3897-910 PMID: 15265986
  51. An anillin homologue, Mid2p, acts during fission yeast cytokinesis to organize the septin ring and promote cell separation.
    J Cell Biol. 2003 Mar 31;160(7):1093-103 PMID: 12668659
  52. Spatial and temporal pathway for assembly and constriction of the contractile ring in fission yeast cytokinesis.
    Dev Cell. 2003 Nov;5(5):723-34 PMID: 14602073
  53. Comparative analysis of cytokinesis in budding yeast, fission yeast and animal cells.
    Curr Biol. 2004 Sep 21;14(18):R806-18 PMID: 15380095
  54. Role of septins and the exocyst complex in the function of hydrolytic enzymes responsible for fission yeast cell separation.
    Mol Biol Cell. 2005 Oct;16(10):4867-81 PMID: 16079182
Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1939-4586
DOI
10.1091/mbc.e07-07-0715
Published
2008-04-00
Epub
2008-00-13
Pages
1680-92
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC2291416
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.e07-07-0715 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2