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PMID: 15385632 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Requirements of fission yeast septins for complex formation, localization, and function.

Molecular biology of the cell ·Vol. 15 ·No. 12 ·2004-12-00 ·Pages 5551-64

An H, Morrell JL, Jennings JL, Link AJ, Gould KL

Abstract

Septins are GTP binding proteins important for cytokinesis in many eukaryotes. The Schizosaccaromyces pombe genome sequence predicts orthologues of four of five Saccharomyces cerevisiae septins involved in cytokinesis and these are named Spns1-4p. That spns1-4 are not essential genes permitted the application of a combined genetic and proteomics approach to determine their functional relationships. Our findings indicate that Spns1-4p are present throughout interphase as a diffusely localized approximately 8.5S complex containing two copies of each septin linked together as a chain in the order Spn3p-Spn4p-Spn1p-Spn2p. Septin recruitment to the medial region of the cell is genetically separable from ring formation, and whereas it is normally restricted to mitosis, it can be promoted without activation of the mitotic cell cycle machinery. Coalescence into ring structures requires Spn1p and Spn4p associate with at least one other septin subunit and the expression of Mid2p that is normally restricted to mitosis. This study establishes the functional requirements for septin complex organization in vivo.

MeSH Terms
Cytoskeletal Proteins/genetics,metabolism GTP-Binding Proteins/genetics,metabolism Interphase Multiprotein Complexes/chemistry,genetics,metabolism Protein Binding Protein Subunits/genetics,metabolism Protein Transport Schizosaccharomyces/cytology,genetics,metabolism Schizosaccharomyces pombe Proteins/genetics,metabolism
Chemicals
Cytoskeletal Proteins Multiprotein Complexes Protein Subunits Schizosaccharomyces pombe Proteins GTP-Binding Proteins Spn1 protein, S pombe Spn2 protein, S pombe Spn3 protein, S pombe Spn4 protein, S pombe
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
An Hanbing
Howard Hughes Medical Institute, Nashville, TN 37232, USA.
Morrell Jennifer L
Jennings Jennifer L
Link Andrew J
Gould Kathleen L
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52 references, click to expand
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e04-07-0640
Published
2004-12-00
Epub
2004-00-22
Pages
5551-64
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC532033
Subset
IM
Grants
NCI NIH HHS · CA098131 · United States
NHLBI NIH HHS · P01 HL068744 · United States
NCI NIH HHS · P50 CA098131 · United States
NIGMS NIH HHS · GM64779 · United States
NIGMS NIH HHS · R01 GM064779 · United States
NIEHS NIH HHS · ES11993 · United States
NINDS NIH HHS · R01 NS043952 · United States
NINDS NIH HHS · NS43952 · United States
NHLBI NIH HHS · HL68744 · United States
NIEHS NIH HHS · R01 ES011993 · United States
External Links
PubMed source DOI: 10.1091/mbc.e04-07-0640 PMC Full Text View journal details
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