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PMID: 15282341 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Protein-protein interactions governing septin heteropentamer assembly and septin filament organization in Saccharomyces cerevisiae.

Molecular biology of the cell ·Vol. 15 ·No. 10 ·2004-10-00 ·Pages 4568-83

Versele M, Gullbrand B, Shulewitz MJ, Cid VJ, Bahmanyar S, Chen RE, Barth P, Alber T, Thorner J

Abstract

Mitotic yeast (Saccharomyces cerevisiae) cells express five related septins (Cdc3, Cdc10, Cdc11, Cdc12, and Shs1) that form a cortical filamentous collar at the mother-bud neck necessary for normal morphogenesis and cytokinesis. All five possess an N-terminal GTPase domain and, except for Cdc10, a C-terminal extension (CTE) containing a predicted coiled coil. Here, we show that the CTEs of Cdc3 and Cdc12 are essential for their association and for the function of both septins in vivo. Cdc10 interacts with a Cdc3-Cdc12 complex independently of the CTE of either protein. In contrast to Cdc3 and Cdc12, the Cdc11 CTE, which recruits the nonessential septin Shs1, is dispensable for its function in vivo. In addition, Cdc11 forms a stoichiometric complex with Cdc12, independent of its CTE. Reconstitution of various multiseptin complexes and electron microscopic analysis reveal that Cdc3, Cdc11, and Cdc12 are all necessary and sufficient for septin filament formation, and presence of Cdc10 causes filament pairing. These data provide novel insights about the connectivity among the five individual septins in functional septin heteropentamers and the organization of septin filaments.

MeSH Terms
Amino Acid Sequence Cell Cycle Proteins/genetics,metabolism Cell Survival Cytoskeletal Proteins/genetics,metabolism GTP Phosphohydrolases Membrane Proteins/chemistry,genetics,metabolism Molecular Sequence Data Multiprotein Complexes Profilins Protein Isoforms/chemistry,genetics,metabolism Protein Structure, Quaternary Recombinant Fusion Proteins/genetics,metabolism Saccharomyces cerevisiae/cytology,metabolism Saccharomyces cerevisiae Proteins/chemistry,genetics,metabolism Schizosaccharomyces pombe Proteins Sequence Alignment Transcription Factors
Chemicals
CDC12 protein, S cerevisiae CDC3 protein, S cerevisiae Cell Cycle Proteins Cytoskeletal Proteins Membrane Proteins Multiprotein Complexes Profilins Protein Isoforms Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins Transcription Factors cdc10 protein, S pombe CDC10 protein, S cerevisiae GTP Phosphohydrolases
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Versele Matthias
Division of Biochemistry and Molecular Biology, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3202, USA.
Gullbrand Björn
Shulewitz Mark J
Cid Victor J
Bahmanyar Shirin
Chen Raymond E
Barth Patrick
Alber Tom
Thorner Jeremy
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e04-04-0330
Published
2004-10-00
Epub
2004-00-28
Pages
4568-83
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC519150
Subset
IM
Grants
NIGMS NIH HHS · R01 GM048958 · United States
NIGMS NIH HHS · R01 GM021841 · United States
NIGMS NIH HHS · GM-48958 · United States
NIGMS NIH HHS · T32 GM007232 · United States
NIGMS NIH HHS · GM-07232 · United States
NIGMS NIH HHS · GM-21841 · United States
External Links
PubMed source DOI: 10.1091/mbc.e04-04-0330 PMC Full Text View journal details
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