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PMID: 18274773 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

Imaging and imagination: understanding the endo-lysosomal system.

Histochemistry and cell biology ·Vol. 129 ·No. 3 ·2008-03-00 ·Pages 253-66

van Meel E, Klumperman J

Abstract

Lysosomes are specialized compartments for the degradation of endocytosed and intracellular material and essential regulators of cellular homeostasis. The importance of lysosomes is illustrated by the rapidly growing number of human disorders related to a defect in lysosomal functioning. Here, we review current insights in the mechanisms of lysosome biogenesis and protein sorting within the endo-lysosomal system. We present increasing evidence for the existence of parallel pathways for the delivery of newly synthesized lysosomal proteins directly from the trans-Golgi network (TGN) to the endo-lysosomal system. These pathways are either dependent or independent of mannose 6-phosphate receptors and likely involve multiple exits for lysosomal proteins from the TGN. In addition, we discuss the different endosomal intermediates and subdomains that are involved in sorting of endocytosed cargo. Throughout our review, we highlight some examples in the literature showing how imaging, especially electron microscopy, has made major contributions to our understanding of the endo-lysosomal system today.

MeSH Terms
Animals Endosomes/metabolism Humans Imagination Lysosomes/metabolism Proteins/metabolism Receptor, IGF Type 2/metabolism trans-Golgi Network/metabolism
Chemicals
Proteins Receptor, IGF Type 2 lysosomal proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
van Meel Eline
Cell Microscopy Center, Department of Cell Biology, University Medical Center Utrecht, Heidelberglaan 100, Utrecht, The Netherlands.
Klumperman Judith
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Article Info
Journal
Histochemistry and cell biology
Abbr.
Histochem Cell Biol
ISSN
0948-6143
DOI
10.1007/s00418-008-0384-0
Published
2008-03-00
Epub
2008-00-15
Pages
253-66
Language
English
Region
Germany
NLM ID
9506663
PMCID
PMC2248605
Subset
IM
External Links
PubMed source DOI: 10.1007/s00418-008-0384-0 PMC Full Text View journal details
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