Home LiteratureArticle Details
PMID: 1831797 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Activation of human monocytes by Streptococcus mutans serotype f polysaccharide: immunoglobulin G Fc receptor expression and tumor necrosis factor and interleukin-1 production.

Infection and immunity ·Vol. 59 ·No. 9 ·1991-09-00 ·Pages 3261-6

Benabdelmoumene S, Dumont S, Petit C, Poindron P, Wachsmann D, Klein JP

Abstract

Streptococcus mutans serotype f polysaccharide (poly f) was prepared from S. mutans whole cells by autoclaving. The poly f was purified by chromatography on DEAE Trisacryl M and Bio-Gel P100, treated with insoluble pronase, and resubjected to chromatography on DEAE Trisacryl M. Normal human blood monocytes, stimulated in vitro with purified poly f, produced extracellular tumor necrosis factor alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) in a dose-dependent fashion as determined by a heterologous two-site sandwich enzyme-linked immunosorbent assay. Poly f also increased the expression of monocyte cell surface receptors for the Fc part of human immunoglobulin G, activity which is correlated with an increase of the phagocytic activity of the stimulated monocytes. Polymyxin B had no effect on TNF-alpha and IL-1 beta release. Neutralization assays with anti-recombinant human TNF-alpha and anti-recombinant human IL-1 beta immunoglobulin G confirmed the fact that the cytotoxic and mitogenic mediators released by the poly f-stimulated monocytes were mainly TNF-alpha and IL-1 beta.

MeSH Terms
Antigens, Differentiation/metabolism Cell Survival Dose-Response Relationship, Immunologic Enzyme-Linked Immunosorbent Assay Humans Interleukin-1/metabolism Lymphocyte Activation/immunology Monocytes/immunology Phagocytosis/immunology Polysaccharides, Bacterial/immunology,isolation & purification Receptors, Fc/metabolism Receptors, IgG Recombinant Proteins Streptococcus mutans/immunology Tumor Necrosis Factor-alpha/metabolism
Chemicals
Antigens, Differentiation Interleukin-1 Polysaccharides, Bacterial Receptors, Fc Receptors, IgG Recombinant Proteins Tumor Necrosis Factor-alpha
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Benabdelmoumene S
Faculté de Chirurgie Dentaire, Unité INSERM 157, Strasbourg, France.
Dumont S
Petit C
Poindron P
Wachsmann D
Klein J P
References (25)
25 references, click to expand
  1. Murine macrophage interleukin-1 release by capsularlike serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans.
    Infect Immun. 1991 Jan;59(1):18-23 PMID: 1987032
  2. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  3. Structure of the serotype f polysaccharide antigen of Streptococcus mutans.
    Carbohydr Res. 1987 Aug 15;166(1):123-31 PMID: 3652074
  4. Induction of tumor necrosis factor-alpha release by lipopolysaccharide and defined lipopolysaccharide partial structures.
    Immunobiology. 1989 Oct;179(4-5):293-307 PMID: 2613271
  5. Structural studies of the serotype-f polysaccharide antigen from Streptococcus mutans OMZ175.
    Infect Immun. 1987 Dec;55(12):3006-10 PMID: 2824381
  6. Human IgG and Streptococcus mutans SR protein contain cross-reactive epitopes.
    J Immunol. 1989 Dec 15;143(12):4257-62 PMID: 2480390
  7. Cytokines in synovial fluid: II. The presence of tumour necrosis factor and interferon.
    Clin Exp Immunol. 1988 Jul;73(1):88-92 PMID: 2458872
  8. Interleukin-1 inducing activity of a streptococcal preparation OK-432 and its fractions by human monocytes.
    J Clin Lab Immunol. 1989 Mar;28(3):129-36 PMID: 2661826
  9. Correlation of plasma interleukin 1 levels with disease activity in rheumatoid arthritis.
    Lancet. 1988 Sep 24;2(8613):706-9 PMID: 2901567
  10. Colorimetric determination of neutral sugars by a resorcinol sulfuric acid micromethod.
    Anal Biochem. 1988 Dec;175(2):525-30 PMID: 3239778
  11. Control of the antitumoral activity of human macrophages produced in large amounts in view of adoptive transfer.
    Eur J Cancer Clin Oncol. 1988 Nov;24(11):1691-8 PMID: 3208813
  12. Role of Streptococcus mutans in human dental decay.
    Microbiol Rev. 1986 Dec;50(4):353-80 PMID: 3540569
  13. Release of tumor necrosis factor (TNF) into mouse peritoneal fluids by OK-432, a streptococcal preparation.
    Immunopharmacology. 1986 Apr;11(2):79-86 PMID: 3710767
  14. Immunochemistry of the Streptococcus mutans BHT cell membrane: detection of determinants cross-reactive with human heart tissue.
    Infect Immun. 1985 May;48(2):280-6 PMID: 3886543
  15. Augmentation of interleukin 1 and interleukin 2 production by OK-432.
    Int J Immunopharmacol. 1985;7(2):263-70 PMID: 3874175
  16. Purification and partial characterization of rat macrophage Fc receptor and binding factor for IgA.
    Biochim Biophys Acta. 1990 Mar 1;1037(3):344-50 PMID: 2310750
  17. Serology and tissue lesions in rabbits immunized with Streptococcus mutans.
    J Immunol. 1983 Dec;131(6):3021-7 PMID: 6417238
  18. Improved method for IgG purification from various animal species by ion exchange chromatography.
    J Immunol Methods. 1984 Jan 20;66(1):75-9 PMID: 6693776
  19. Utilization of the biotin/avidin system to amplify the sensitivity of the enzyme-linked immunosorbent assay (ELISA).
    J Immunol Methods. 1983 Feb 11;56(3):329-39 PMID: 6833765
  20. Lymphoid cell responses to bacterial cell wall components: polyclonal and immune responses of murine B cells to Streptococcus mutans carbohydrate antigens.
    J Immunol. 1981 Nov;127(5):2106-12 PMID: 7028871
  21. Binding of streptococcal antigens to muscle tissue in vitro.
    Infect Immun. 1980 Feb;27(2):604-13 PMID: 6991420
  22. Evidence for an immunological relationship between Streptococcus mutans and human cardiac tissue.
    Infect Immun. 1980 Feb;27(2):576-88 PMID: 6991419
  23. Characterization of lymphocyte-activating factor (LAF) produced by the macrophage cell line, P388D1. I. Enhancement of LAF production by activated T lymphocytes.
    J Immunol. 1978 May;120(5):1497-503 PMID: 149171
  24. Chemical and immunological properties of the type f polysaccharide antigen of Streptococcus mutans.
    Infect Immun. 1976 Jul;14(1):203-11 PMID: 59704
  25. Serum and salivary antibody responses in rats orally immunized with Streptococcus mutans carbohydrate protein conjugate associated with liposomes.
    Infect Immun. 1986 May;52(2):408-13 PMID: 3699888
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.59.9.3261-3266.1991
Published
1991-09-00
Pages
3261-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC258161
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.59.9.3261-3266.1991 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2