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PMID: 18329872 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Review

On helicases and other motor proteins.

Current opinion in structural biology ·Vol. 18 ·No. 2 ·2008-04-00 ·Pages 243-57

Enemark EJ, Joshua-Tor L

Abstract

Helicases are molecular machines that utilize energy derived from ATP hydrolysis to move along nucleic acids and to separate base-paired nucleotides. The movement of the helicase can also be described as a stationary helicase that pumps nucleic acid. Recent structural data for the hexameric E1 helicase of papillomavirus in complex with single-stranded DNA and MgADP has provided a detailed atomic and mechanistic picture of its ATP-driven DNA translocation. The structural and mechanistic features of this helicase are compared with the hexameric helicase prototypes T7gp4 and SV40 T-antigen. The ATP-binding site architectures of these proteins are structurally similar to the sites of other prototypical ATP-driven motors such as F1-ATPase, suggesting related roles for the individual site residues in the ATPase activity.

MeSH Terms
Adenosine Triphosphate/metabolism Amino Acid Sequence Animals Binding Sites DNA/chemistry,metabolism DNA Helicases/chemistry,metabolism Molecular Motor Proteins/metabolism Molecular Sequence Data RNA Helicases/chemistry,metabolism
Chemicals
Molecular Motor Proteins Adenosine Triphosphate DNA DNA Helicases RNA Helicases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Enemark Eric J
W.M. Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, United States.
Joshua-Tor Leemor
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Article Info
Journal
Current opinion in structural biology
Abbr.
Curr Opin Struct Biol
ISSN
0959-440X
DOI
10.1016/j.sbi.2008.01.007
Published
2008-04-00
Epub
2008-00-10
Pages
243-57
Language
English
Region
England
NLM ID
9107784
PMCID
PMC2396192
Subset
IM
Grants
NIAID NIH HHS · R01 AI046724 · United States
NIAID NIH HHS · R01 AI046724-06A2 · United States
NIAID NIH HHS · AI146724 · United States
External Links
PubMed source DOI: 10.1016/j.sbi.2008.01.007 PMC Full Text View journal details
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