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PMID: 1833617 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of Escherichia coli genes whose expression increases as a function of external pH.

Molecular & general genetics : MGG ·Vol. 229 ·No. 2 ·1991-10-00 ·Pages 197-205

Heyde M, Coll JL, Portalier R

Abstract

Using transposon TnphoA and a plate screening method, we have isolated a set of Escherichia coli strains carrying phoA fusions with genes whose expression is modulated as a function of external pH. Besides fusions with the ompF gene and the malB locus, thirteen independent fusions were analysed whose expression is maximal during growth at pHs ranging from 7.0 to 8.5 and minimal during growth at pH 5.0. Six different genetic loci, called phmA, phmB, phmC, phmD, phmE and phmF (for pH modulated) were characterized and localized on the E. coli chromosome at approx. 12, 18, 41, 45, 75 and 84 min, respectively. Expression of phmA::phoA fusions is also influenced when internal pH or environmental conditions such as osmolarity or anaerobiosis are modified. EnvZ protein is not involved in the regulation of phm::phoA fusions.

Related Genes
malB ompF phm phoA
MeSH Terms
Bacteriophage lambda/genetics Electrophoresis, Polyacrylamide Gel Escherichia coli/genetics Genes, Bacterial Genes, Viral Hydrogen-Ion Concentration Osmolar Concentration
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Heyde M
Laboratoire de Microbiologie et de Génétique Moléculaire (UMR CNRS 106), Université Claude Bernard Lyon I, Villeurbanne, France.
Coll J L
Portalier R
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00272156
Published
1991-10-00
Pages
197-205
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00272156
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