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PMID: 18368500 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Complexity of Hsp90 in organelle targeting.

Plant molecular biology ·Vol. 67 ·No. 4 ·2008-07-00 ·Pages 323-34

Prassinos C, Haralampidis K, Milioni D, Samakovli D, Krambis K, Hatzopoulos P

Abstract

Heat shock protein 90 (Hsp90) is an abundant and highly conserved molecular chaperone. In Arabidopsis, the Hsp90 gene family consists of seven members. Here, we report that the AtHsp90-6 gene gives rise to two mRNA populations, termed AtHsp90-6L and AtHsp90-6S due to alternative initiation of transcription. The AtHsp90-6L and AtHsp90-6S transcription start sites are located 228 nucleotides upstream and 124 nucleotides downstream of the annotated translation start site, respectively. Both transcripts are detected under normal or heat-shock conditions. The inducibility of AtHsp90-6 mRNAs by heat shock implies a potential role of both isoforms in stress management. Stable transformation experiments with fusion constructs between the N-terminal part of each AtHsp90-6 isoform and green fluorescent protein indicated import of both fusion proteins into mitochondria. In planta investigation confirmed that fusion of the AtHsp90-5 N-terminus to green fluorescent protein (GFP) did result in specific chloroplastic localization. The mechanisms of regulation for mitochondria- and plastid-localized chaperone-encoding genes are not well understood. Future work is needed to address the possible roles of harsh environmental conditions and developmental processes on fine-tuning and compartmentalization of the AtHsp90-6L, AtHsp90-6S, and AtHsp90-5 proteins in Arabidopsis.

MeSH Terms
Alternative Splicing Amino Acid Sequence Arabidopsis/metabolism Base Sequence DNA Primers HSP90 Heat-Shock Proteins/chemistry,genetics,metabolism,physiology Microscopy, Fluorescence Molecular Sequence Data Organelles/metabolism RNA, Messenger/genetics Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Transcription, Genetic
Chemicals
DNA Primers HSP90 Heat-Shock Proteins RNA, Messenger
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Prassinos Constantinos
Laboratory of Molecular Biology, Agricultural Biotechnology Department, Agricultural University of Athens, Athens, Greece.
Haralampidis Kosmas
Milioni Dimitra
Samakovli Despina
Krambis Konstantinos
Hatzopoulos Polydefkis
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/s11103-008-9322-8
Published
2008-07-00
Epub
2008-00-27
Pages
323-34
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/s11103-008-9322-8 View journal details
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