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PMID: 18443223 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

CENP-E combines a slow, processive motor and a flexible coiled coil to produce an essential motile kinetochore tether.

The Journal of cell biology ·Vol. 181 ·No. 3 ·2008-05-05 ·Pages 411-9

Kim Y, Heuser JE, Waterman CM, Cleveland DW

Abstract

The mitotic kinesin centromere protein E (CENP-E) is an essential kinetochore component that directly contributes to the capture and stabilization of spindle microtubules by kinetochores. Although reduction in CENP-E leads to high rates of whole chromosome missegregation, neither its properties as a microtubule-dependent motor nor how it contributes to the dynamic linkage between kinetochores and microtubules is known. Using single-molecule assays, we demonstrate that CENP-E is a very slow, highly processive motor that maintains microtubule attachment for long periods. Direct visualization of full-length Xenopus laevis CENP-E reveals a highly flexible 230-nm coiled coil separating its kinetochore-binding and motor domains. We also show that full-length CENP-E is a slow plus end-directed motor whose activity is essential for metaphase chromosome alignment. We propose that the highly processive microtubule-dependent motor activity of CENP-E serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules.

MeSH Terms
Animals Chromosomal Proteins, Non-Histone/chemistry,genetics,metabolism Chromosome Segregation Kinetochores/chemistry,metabolism Microtubules/chemistry,metabolism Models, Biological Molecular Motor Proteins/chemistry,genetics,metabolism Protein Structure, Secondary Recombinant Fusion Proteins/chemistry,genetics,metabolism Spindle Apparatus/chemistry,metabolism Xenopus Proteins/chemistry,genetics,metabolism Xenopus laevis
Chemicals
Chromosomal Proteins, Non-Histone Molecular Motor Proteins Recombinant Fusion Proteins Xenopus Proteins centromere protein E
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kim Yumi
Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, CA 92093, USA.
Heuser John E
Waterman Clare M
Cleveland Don W
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
1540-8140
DOI
10.1083/jcb.200802189
Published
2008-05-05
Epub
2008-00-28
Pages
411-9
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2364708
Subset
IM
Grants
NIGMS NIH HHS · R01 GM029513 · United States
NIGMS NIH HHS · R37 GM029513 · United States
NIGMS NIH HHS · GM29513 · United States
External Links
PubMed source DOI: 10.1083/jcb.200802189 PMC Full Text View journal details
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