Home LiteratureArticle Details
PMID: 17435749 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

The outer plate in vertebrate kinetochores is a flexible network with multiple microtubule interactions.

Nature cell biology ·Vol. 9 ·No. 5 ·2007-05-00 ·Pages 516-22

Dong Y, Vanden Beldt KJ, Meng X, Khodjakov A, McEwen BF

Abstract

Intricate interactions between kinetochores and microtubules are essential for the proper distribution of chromosomes during mitosis. A crucial long-standing question is how vertebrate kinetochores generate chromosome motion while maintaining attachments to the dynamic plus ends of the multiple kinetochore MTs (kMTs) in a kinetochore fibre. Here, we demonstrate that individual kMTs in PtK(1) cells are attached to the kinetochore outer plate by several fibres that either embed the microtubule plus-end tips in a radial mesh, or extend out from the outer plate to bind microtubule walls. The extended fibres also interact with the walls of nearby microtubules that are not part of the kinetochore fibre. These structural data, in combination with other recent reports, support a network model of kMT attachment wherein the fibrous network in the unbound outer plate, including the Hec1-Ndc80 complex, dissociates and rearranges to form kMT attachments.

MeSH Terms
Animals Binding Sites Cell Line Epithelial Cells/metabolism,ultrastructure Kinetochores/metabolism,ultrastructure Microscopy, Electron, Transmission Microtubule-Associated Proteins/metabolism Microtubules/metabolism,ultrastructure Mitosis/physiology Models, Molecular Nuclear Proteins/metabolism Protein Binding Protein Conformation Tomography, X-Ray Computed Vertebrates
Chemicals
Microtubule-Associated Proteins Nuclear Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Dong Yimin
Department of Biomedical Sciences, School of Public Health, State University of New York at Albany, Albany, NY 12201, USA.
Vanden Beldt Kristin J
Meng Xing
Khodjakov Alexey
McEwen Bruce F
References (32)
32 references, click to expand
  1. Merotelic kinetochore orientation is a major mechanism of aneuploidy in mitotic mammalian tissue cells.
    J Cell Biol. 2001 Apr 30;153(3):517-27 PMID: 11331303
  2. The conserved KMN network constitutes the core microtubule-binding site of the kinetochore.
    Cell. 2006 Dec 1;127(5):983-97 PMID: 17129783
  3. Simple centromere, complex kinetochore: linking spindle microtubules and centromeric DNA in budding yeast.
    J Cell Biol. 2002 Apr 15;157(2):199-203 PMID: 11956223
  4. Use of frozen-hydrated axonemes to assess imaging parameters and resolution limits in cryoelectron tomography.
    J Struct Biol. 2002 Apr-May;138(1-2):47-57 PMID: 12160700
  5. Chromosome-microtubule interactions during mitosis.
    Annu Rev Cell Dev Biol. 2002;18:193-219 PMID: 12142285
  6. Centromeres and kinetochores: from epigenetics to mitotic checkpoint signaling.
    Cell. 2003 Feb 21;112(4):407-21 PMID: 12600307
  7. Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes.
    Genes Dev. 2003 Dec 1;17(23):2902-21 PMID: 14633972
  8. A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension.
    Genes Dev. 2004 Sep 15;18(18):2255-68 PMID: 15371340
  9. The dynamic kinetochore-microtubule interface.
    J Cell Sci. 2004 Nov 1;117(Pt 23):5461-77 PMID: 15509863
  10. The fine structure of the kinetochore of a mammalian cell in vitro.
    Chromosoma. 1966;19(1):28-43 PMID: 5912064
  11. The structure of the cold-stable kinetochore fiber in metaphase PtK1 cells.
    Chromosoma. 1981;84(1):145-58 PMID: 7297248
  12. The centromere-kinetochore complex: a repeat subunit model.
    J Cell Biol. 1991 Jun;113(5):1091-110 PMID: 1828250
  13. Structure of the colcemid-treated PtK1 kinetochore outer plate as determined by high voltage electron microscopic tomography.
    J Cell Biol. 1993 Jan;120(2):301-12 PMID: 8421050
  14. Chromosome fragments possessing only one kinetochore can congress to the spindle equator.
    J Cell Biol. 1997 Jan 27;136(2):229-40 PMID: 9015296
  15. Kinetochore fiber maturation in PtK1 cells and its implications for the mechanisms of chromosome congression and anaphase onset.
    J Cell Biol. 1997 Jun 30;137(7):1567-80 PMID: 9199171
  16. Microtubule polymerization dynamics.
    Annu Rev Cell Dev Biol. 1997;13:83-117 PMID: 9442869
  17. The vertebrate cell kinetochore and its roles during mitosis.
    Trends Cell Biol. 1998 Aug;8(8):310-8 PMID: 9704407
  18. A new look at kinetochore structure in vertebrate somatic cells using high-pressure freezing and freeze substitution.
    Chromosoma. 1998 Dec;107(6-7):366-75 PMID: 9914368
  19. Formation of a dynamic kinetochore- microtubule interface through assembly of the Dam1 ring complex.
    Mol Cell. 2005 Jan 21;17(2):277-90 PMID: 15664196
  20. Hec1 and nuf2 are core components of the kinetochore outer plate essential for organizing microtubule attachment sites.
    Mol Biol Cell. 2005 Feb;16(2):519-31 PMID: 15548592
  21. The yeast DASH complex forms closed rings on microtubules.
    Nat Struct Mol Biol. 2005 Feb;12(2):138-43 PMID: 15640796
  22. Molecular organization of the Ndc80 complex, an essential kinetochore component.
    Proc Natl Acad Sci U S A. 2005 Apr 12;102(15):5363-7 PMID: 15809444
  23. Architecture of the human ndc80-hec1 complex, a critical constituent of the outer kinetochore.
    J Biol Chem. 2005 Aug 12;280(32):29088-95 PMID: 15961401
  24. Measuring the stoichiometry and physical interactions between components elucidates the architecture of the vertebrate kinetochore.
    Mol Biol Cell. 2005 Oct;16(10):4882-92 PMID: 16079178
  25. Kinetochore capture and bi-orientation on the mitotic spindle.
    Nat Rev Mol Cell Biol. 2005 Dec;6(12):929-42 PMID: 16341079
  26. Chromosomes can congress to the metaphase plate before biorientation.
    Science. 2006 Jan 20;311(5759):388-91 PMID: 16424343
  27. The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres.
    Nat Cell Biol. 2006 May;8(5):446-57 PMID: 16622420
  28. Molecular architecture of a kinetochore-microtubule attachment site.
    Nat Cell Biol. 2006 Jun;8(6):581-5 PMID: 16715078
  29. Kinetochores use a novel mechanism for coordinating the dynamics of individual microtubules.
    Curr Biol. 2006 Jun 20;16(12):1217-23 PMID: 16782013
  30. CENP-F is a novel microtubule-binding protein that is essential for kinetochore attachments and affects the duration of the mitotic checkpoint delay.
    Chromosoma. 2006 Aug;115(4):320-9 PMID: 16601978
  31. Kinetochore microtubule dynamics and attachment stability are regulated by Hec1.
    Cell. 2006 Dec 1;127(5):969-82 PMID: 17129782
  32. CENP-E is essential for reliable bioriented spindle attachment, but chromosome alignment can be achieved via redundant mechanisms in mammalian cells.
    Mol Biol Cell. 2001 Sep;12(9):2776-89 PMID: 11553716
Article Info
Journal
Nature cell biology
Abbr.
Nat Cell Biol
ISSN
1465-7392
DOI
10.1038/ncb1576
Published
2007-05-00
Epub
2007-00-15
Pages
516-22
Language
English
Region
England
NLM ID
100890575
PMCID
PMC2895818
Subset
IM
Grants
NCRR NIH HHS · P41 RR01219 · United States
NIGMS NIH HHS · R01 GM066270-05A1 · United States
NIGMS NIH HHS · R01 GM06627 · United States
NIGMS NIH HHS · GM59363 · United States
NIGMS NIH HHS · R01 GM059363 · United States
NCRR NIH HHS · P41 RR001219 · United States
NIGMS NIH HHS · R01 GM059363-09 · United States
NIGMS NIH HHS · R01 GM066270 · United States
External Links
PubMed source DOI: 10.1038/ncb1576 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2