Absence of 5' terminal capping in encephalomyocarditis virus RNA.

Nucleic acids research ·Vol. 3 ·No. 10 ·1976-10-00 ·Pages 2771-87

Frisby D, Eaton M, Fellner P

Abstract

The nature of the 5' terminus of encephalomyocarditis (EMC) virion RNA has been investigated. We have failed to detect any capped products or nucleoside polyphosphates arising upon complete digestion of the RNA with T1, T2, and pancreatic ribonucleases, and it would therefore appear that the 5' terminus of EMC virus RNA is not phosphorylated and not capped with m7G. EMC virions do contain, however, large amounts of all four 5'-monosubstituted nucleoside triphosphates (4.2M pppG; 16.4M pppA; 3.OM pppU and 2.5M pppC), of which at least a proportion (about 15-20%) appear to remain bound to fully denatured RNA in the presence of divalent cations.

MeSH Terms

Base Sequence Encephalomyocarditis virus/analysis Guanine Nucleotides/analysis Nucleotides/analysis RNA, Messenger/analysis RNA, Viral

Chemicals

Guanine Nucleotides Nucleotides RNA, Messenger RNA, Viral

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Frisby D

Eaton M

Fellner P

References (30)

30 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/3.10.2771

Published

1976-10-00

Pages

2771-87

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343127

Subset

External Links

PubMed source DOI: 10.1093/nar/3.10.2771 PMC Full Text View journal details

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Absence of 5' terminal capping in encephalomyocarditis virus RNA.

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