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PMID: 1868828 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Two conserved domains in the NGF propeptide are necessary and sufficient for the biosynthesis of correctly processed and biologically active NGF.

The EMBO journal ·Vol. 10 ·No. 9 ·1991-09-00 ·Pages 2395-400

Suter U, Heymach JV, Shooter EM

Abstract

The three members of the neurotrophin family (NGF, BDNF and NT-3) are synthesized as large precursor proteins which undergo proteolytic processing to yield biologically active, mature neurotrophic factors. We have used in vitro mutagenesis to examine the pro-region in the NGF precursor protein as a first step towards a general understanding of the role of propeptides in the biosynthesis of neurotrophins. Our results demonstrate that only two small domains within the NGF propeptide are required for the expression and secretion of properly processed and biologically active, recombinant mouse NGF in COS-7 cells. Domain I plays an important role in the expression of active NGF while domain II is involved in proteolytic processing. Both domains are partially conserved between the propeptides of NGF proteins isolated from different species as well as BDNF and NT-3.

MeSH Terms
Amino Acid Sequence Animals Cell Line Chromosome Deletion Cloning, Molecular DNA/genetics Electrophoresis, Polyacrylamide Gel Hydrolysis Mice Molecular Sequence Data Mutagenesis Nerve Growth Factors/biosynthesis,metabolism Protein Precursors/biosynthesis,metabolism Protein Processing, Post-Translational Protein Sorting Signals/metabolism
Chemicals
Nerve Growth Factors Protein Precursors Protein Sorting Signals DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Suter U
Department of Neurobiology, Stanford University School of Medicine, CA 94305-5401.
Heymach J V
Shooter E M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-09-00
Pages
2395-400
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452934
Subset
IM
Grants
NINDS NIH HHS · NS04270 · United States
External Links
PubMed source PMC Full Text View journal details
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