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PMID: 1871136 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Prevention of human immunodeficiency virus type 1 integrase expression in Escherichia coli by a ribozyme.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 16 ·1991-08-15 ·Pages 7303-7

Sioud M, Drlica K

Abstract

Ribozymes are potentially very powerful agents for perturbing intracellular gene expression. However, pilot experiments in eukaryotes have met with mixed success. We now report that a ribozyme designed to cleave the integrase gene of the human immunodeficiency virus (HIV), when transcribed from a plasmid in Escherichia coli, led to destruction of integrase RNA and complete blockage of integrase protein synthesis. These results indicate that ribozymes can be used to study intracellular gene expression in bacteria and that the HIV-1 integrase gene may be a useful target for therapeutic ribozymes.

MeSH Terms
Base Sequence Cloning, Molecular DNA Nucleotidyltransferases/antagonists & inhibitors,biosynthesis,genetics Escherichia coli/enzymology,genetics HIV-1/enzymology,genetics Integrases Kinetics Models, Structural Molecular Sequence Data Nucleic Acid Conformation Plasmids RNA, Catalytic/metabolism Recombinant Proteins/antagonists & inhibitors,biosynthesis
Chemicals
RNA, Catalytic Recombinant Proteins DNA Nucleotidyltransferases Integrases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sioud M
Public Health Research Institute, New York, NY 10016.
Drlica K
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.16.7303
Published
1991-08-15
Pages
7303-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC52283
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.88.16.7303 PMC Full Text View journal details
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