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PMID: 18757556 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The cold-inducible CBF1 factor-dependent signaling pathway modulates the accumulation of the growth-repressing DELLA proteins via its effect on gibberellin metabolism.

The Plant cell ·Vol. 20 ·No. 8 ·2008-08-00 ·Pages 2117-29

Achard P, Gong F, Cheminant S, Alioua M, Hedden P, Genschik P

Abstract

Plants have evolved robust mechanisms to respond and adapt to unfavorable environmental conditions, such as low temperature. The C-repeat/drought-responsive element binding factor CBF1/DREB1b gene encodes a transcriptional activator transiently induced by cold that controls the expression of a set of genes responding to low temperature (the CBF regulon). Constitutive expression of CBF1 confers freezing tolerance but also slows growth. Here, we propose that low temperature-induced CBF1 expression restrains growth at least in part by allowing the accumulation of DELLAs, a family of nuclear growth-repressing proteins, the degradation of which is stimulated by gibberellin (GA). We show that cold/CBF1 enhances the accumulation of a green fluorescent protein (GFP)-tagged DELLA protein (GFP-RGA) by reducing GA content through stimulating expression of GA-inactivating GA 2-oxidase genes. Accordingly, transgenic plants that constitutively express CBF1 accumulate less bioactive GA and as a consequence exhibit dwarfism and late flowering. Both phenotypes are suppressed when CBF1 is expressed in a line lacking two DELLA proteins, GA-INSENSITIVE and REPRESSOR OF GA1-3. In addition, we show that DELLAs contribute significantly to CBF1-induced cold acclimation and freezing tolerance by a mechanism that is distinct from the CBF regulon. We conclude that DELLAs are components of the CBF1-mediated cold stress response.

MeSH Terms
Arabidopsis/genetics,growth & development,metabolism Arabidopsis Proteins/genetics,metabolism,physiology Cold Temperature Gene Expression Regulation, Plant Gibberellins/metabolism Immunoblotting Reverse Transcriptase Polymerase Chain Reaction Signal Transduction/genetics,physiology Trans-Activators/genetics,metabolism,physiology
Chemicals
Arabidopsis Proteins CBF1 protein, Arabidopsis Gibberellins Trans-Activators
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Achard Patrick
Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, Unité Propre de Recherche 2357, Conventioné avec l'Université Louis Pasteur, 67084 Strasbourg, France.
Gong Fan
Cheminant Soizic
Alioua Malek
Hedden Peter
Genschik Pascal
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.108.058941
Published
2008-08-00
Epub
2008-00-29
Pages
2117-29
Language
English
Region
England
NLM ID
9208688
PMCID
PMC2553604
Subset
IM
Grants
Biotechnology and Biological Sciences Research Council · BBS/E/C/00004161 · United Kingdom
Biotechnology and Biological Sciences Research Council · BBS/E/C/00004162 · United Kingdom
Biotechnology and Biological Sciences Research Council · BBS/E/C/00004954 · United Kingdom
External Links
PubMed source DOI: 10.1105/tpc.108.058941 PMC Full Text View journal details
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