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PMID: 18791237 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A novel septin-associated protein, Syp1p, is required for normal cell cycle-dependent septin cytoskeleton dynamics in yeast.

Genetics ·Vol. 180 ·No. 3 ·2008-11-00 ·Pages 1445-57

Qiu W, Neo SP, Yu X, Cai M

Abstract

Septins are a family of GTP-binding proteins whose heterooligomeric complex is the basic structural element of the septin filaments found in many eukaryotic organisms. In budding yeast, septins are mainly confined at the mother-daughter junction and are required for cell morphogenesis and division. Septins undergo assembly and disassembly in accordance with the progression of the cell cycle. In this report, we identified the yeast protein Syp1p as a new regulator of septin dynamics. Syp1p colocalizes with septins throughout most of the cell cycle. Syp1p interacts with the septin subunit Cdc10p and can be precipitated by Cdc10p and Cdc12p. In the syp1Delta mutant, both formation of a complete septin ring at the incipient bud site and disassembly of the septin ring in later stages of cell division are significantly delayed. In addition, overexpression of Syp1p causes marked acceleration of septin disassembly. The fluorescence recovery after photobleaching (FRAP) assay further showed that Syp1p promotes septin turnover in different cell cycle stages. These results suggest that Syp1p is involved in the regulation of cell cycle-dependent dynamics of the septin cytoskeleton in yeast.

MeSH Terms
Cell Cycle/physiology Cell Cycle Proteins/genetics,metabolism Cell Division Cellular Structures Cytoskeletal Proteins/genetics,metabolism Cytoskeleton/physiology Fluorescence Recovery After Photobleaching GTP Phosphohydrolases/genetics,metabolism Gene Expression Regulation, Fungal Green Fluorescent Proteins/metabolism Membrane Proteins/genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Schizosaccharomyces/genetics Two-Hybrid System Techniques
Chemicals
CDC12 protein, S cerevisiae Cell Cycle Proteins Cytoskeletal Proteins Membrane Proteins Saccharomyces cerevisiae Proteins Green Fluorescent Proteins CDC10 protein, S cerevisiae GTP Phosphohydrolases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Qiu Wenjie
Institute of Molecular and Cell Biology, Singapore 138673, Republic of Singapore.
Neo Suat Peng
Yu Xianwen
Cai Mingjie
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.108.091900
Published
2008-11-00
Epub
2008-00-14
Pages
1445-57
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC2581947
Subset
IM
External Links
PubMed source DOI: 10.1534/genetics.108.091900 PMC Full Text View journal details
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