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PMID: 18832349 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

The spindle positioning protein Kar9p interacts with the sumoylation machinery in Saccharomyces cerevisiae.

Genetics ·Vol. 180 ·No. 4 ·2008-12-00 ·Pages 2033-55

Meednu N, Hoops H, D'Silva S, Pogorzala L, Wood S, Farkas D, Sorrentino M, Sia E, Meluh P, Miller RK

Abstract

Accurate positioning of the mitotic spindle is important for the genetic material to be distributed evenly in dividing cells, but little is known about the mechanisms that regulate this process. Here we report that two microtubule-associated proteins important for spindle positioning interact with several proteins in the sumoylation pathway. By two-hybrid analysis, Kar9p and Bim1p interact with the yeast SUMO Smt3p, the E2 enzyme Ubc9p, an E3 Nfi1p, as well as Wss1p, a weak suppressor of a temperature-sensitive smt3 allele. The physical interaction between Kar9p and Ubc9p was confirmed by in vitro binding assays. A single-amino-acid substitution in Kar9p, L304P disrupted its two-hybrid interaction with proteins in the sumoylation pathway, but retained its interactions with the spindle positioning proteins Bim1p, Stu2p, Bik1p, and Myo2p. The kar9-L304P mutant showed defects in positioning the mitotic spindle, with the spindle located more distally than normal. Whereas wild-type Kar9p-3GFP normally localizes to only the bud-directed spindle pole body (SPB), Kar9p-L304P-3GFP was mislocalized to both SPBs. Using a reconstitution assay, Kar9p was sumoylated in vitro. We propose a model in which sumoylation regulates spindle positioning by restricting Kar9p to one SPB. These findings raise the possibility that sumoylation could regulate other microtubule-dependent processes.

MeSH Terms
Binding Sites Cell Cycle Proteins/genetics,metabolism Microscopy, Fluorescence Microtubule Proteins/genetics,metabolism Mutation Nuclear Proteins/genetics,metabolism Phosphorylation Repressor Proteins/genetics,metabolism Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Small Ubiquitin-Related Modifier Proteins/genetics,metabolism Spindle Apparatus/metabolism
Chemicals
BIM1 protein, S cerevisiae Cell Cycle Proteins KAR9 protein, S cerevisiae Microtubule Proteins Nuclear Proteins Repressor Proteins SMT3 protein, S cerevisiae Saccharomyces cerevisiae Proteins Small Ubiquitin-Related Modifier Proteins
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Meednu Nida
Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA.
Hoops Harold
D'Silva Sonia
Pogorzala Leah
Wood Schuyler
Farkas David
Sorrentino Mark
Sia Elaine
Meluh Pam
Miller Rita K
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.108.095042
Published
2008-12-00
Epub
2008-00-01
Pages
2033-55
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC2600940
Subset
IM
External Links
PubMed source DOI: 10.1534/genetics.108.095042 PMC Full Text View journal details
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