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PMID: 10579719 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cell cycle-regulated attachment of the ubiquitin-related protein SUMO to the yeast septins.

The Journal of cell biology ·Vol. 147 ·No. 5 ·1999-11-29 ·Pages 981-94

Johnson ES, Blobel G

Abstract

SUMO is a ubiquitin-related protein that functions as a posttranslational modification on other proteins. SUMO conjugation is essential for viability in Saccharomyces cerevisiae and is required for entry into mitosis. We have found that SUMO is attached to the septins Cdc3, Cdc11, and Shs1/Sep7 specifically during mitosis, with conjugates appearing shortly before anaphase onset and disappearing abruptly at cytokinesis. Septins are components of a belt of 10-nm filaments encircling the yeast bud neck. Intriguingly, only septins on the mother cell side of the bud neck are sumoylated. We have identified four major SUMO attachment-site lysine residues in Cdc3, one in Cdc11, and two in Shs1, all within the consensus sequence (IVL)KX(ED). Mutating these sites eliminated the vast majority of bud neck-associated SUMO, as well as the bulk of total SUMO conjugates in G(2)/M-arrested cells, indicating that sumoylated septins are the most abundant SUMO conjugates at this point in the cell cycle. This mutant has a striking defect in disassembly of septin rings, resulting in accumulation of septin rings marking previous division sites. Thus, SUMO conjugation plays a role in regulating septin ring dynamics during the cell cycle.

MeSH Terms
Binding Sites/genetics,physiology Cell Cycle/genetics,physiology Cell Cycle Proteins/genetics,metabolism DNA Mutational Analysis Fungal Proteins/genetics,metabolism Lysine/genetics,metabolism Profilins SUMO-1 Protein Saccharomyces cerevisiae/cytology,genetics,physiology Saccharomyces cerevisiae Proteins Ubiquitins/genetics,metabolism
Chemicals
CDC3 protein, S cerevisiae Cell Cycle Proteins Fungal Proteins Profilins SUMO-1 Protein Saccharomyces cerevisiae Proteins Ubiquitins Lysine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Johnson E S
Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, New York 10021, USA. [email protected]
Blobel G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.147.5.981
Email
[email protected]
Published
1999-11-29
Pages
981-94
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2169351
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.147.5.981 PMC Full Text View journal details
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