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PMID: 1886619 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

High-frequency transfer of linear DNA containing 5'-covalently linked terminal proteins: electroporation of bacteriophage PRD1 genome into Escherichia coli.

Molecular & general genetics : MGG ·Vol. 228 ·No. 1-2 ·1991-08-00 ·Pages 65-9

Lyra C, Savilahti H, Bamford DH

Abstract

Using electroporation with the phage PRD1 genome, we set up a high-frequency DNA transfer system for a linear dsDNA molecule with 5'-covalently linked terminal proteins. The transfer was saturated when more than 100 ng of PRD1 genome was used. Electroporation efficiency was about four orders of magnitude higher than that obtained with transfection. Removal of the terminal protein abolished plaque formation, which could not be rescued by supplying the terminal protein or phage DNA polymerase or both in trans.

Related Genes

amp bla cam kan tet tet(am)

MeSH Terms

Bacteriophages/genetics DNA, Recombinant DNA, Viral/isolation & purification Escherichia coli/genetics Genetic Markers Plasmids Transfection

Chemicals

DNA, Recombinant DNA, Viral Genetic Markers

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Lyra C

Department of Genetics, University of Helsinki, Finland.

Savilahti H

Bamford D H

References (28)

28 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00282449

Published

1991-08-00

Pages

65-9

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00282449

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High-frequency transfer of linear DNA containing 5'-covalently linked terminal proteins: electroporation of bacteriophage PRD1 genome into Escherichia coli.

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