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PMID: 18985028 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Hepatitis C virus infection protein network.

Molecular systems biology ·Vol. 4 ·2008-00-00 ·Pages 230

de Chassey B, Navratil V, Tafforeau L, Hiet MS, Aublin-Gex A, Agaugué S, Meiffren G, Pradezynski F, Faria BF, Chantier T, Le Breton M, Pellet J, Davoust N, Mangeot PE, Chaboud A, Penin F, Jacob Y, Vidalain PO, Vidal M, André P, Rabourdin-Combe C, Lotteau V

Abstract

A proteome-wide mapping of interactions between hepatitis C virus (HCV) and human proteins was performed to provide a comprehensive view of the cellular infection. A total of 314 protein-protein interactions between HCV and human proteins was identified by yeast two-hybrid and 170 by literature mining. Integration of this data set into a reconstructed human interactome showed that cellular proteins interacting with HCV are enriched in highly central and interconnected proteins. A global analysis on the basis of functional annotation highlighted the enrichment of cellular pathways targeted by HCV. A network of proteins associated with frequent clinical disorders of chronically infected patients was constructed by connecting the insulin, Jak/STAT and TGFbeta pathways with cellular proteins targeted by HCV. CORE protein appeared as a major perturbator of this network. Focal adhesion was identified as a new function affected by HCV, mainly by NS3 and NS5A proteins.

MeSH Terms
Hepacivirus/metabolism,physiology Hepatitis C/metabolism Humans Protein Binding Two-Hybrid System Techniques Viral Proteins/metabolism
Chemicals
Viral Proteins
Authors & Affiliations
22 authors, click to expand affiliations / ORCID
de Chassey B
IMAP Team, Inserm Unit 851, Lyon, France.
Navratil V
Tafforeau L
Hiet M S
Aublin-Gex A
Agaugué S
Meiffren G
Pradezynski F
Faria B F
Chantier T
Le Breton M
Pellet J
Davoust N
Mangeot P E
Chaboud A
Penin F
Jacob Y
Vidalain P O
Vidal M
André P
Rabourdin-Combe C
Lotteau V
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Article Info
Journal
Molecular systems biology
Abbr.
Mol Syst Biol
ISSN
1744-4292
DOI
10.1038/msb.2008.66
Published
2008-00-00
Epub
2008-00-04
Pages
230
Language
English
Region
England
NLM ID
101235389
PMCID
PMC2600670
Subset
IM
External Links
PubMed source DOI: 10.1038/msb.2008.66 PMC Full Text View journal details
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