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PMID: 1898660 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Histologic transformation of follicular lymphoma to diffuse lymphoma represents tumor progression by a single malignant B cell.

The Journal of experimental medicine ·Vol. 173 ·No. 1 ·1991-01-01 ·Pages 197-207

Zelenetz AD, Chen TT, Levy R

Abstract

To investigate the clonal relationship between follicular lymphoma (FL) and transformed diffuse lymphoma (tDL), we examined the expression of tumor idiotype, immunoglobulin (Ig) gene rearrangements and sequence of Ig variable genes in paired tissue specimens. All 16 cases analyzed expressed surface immunoglobulin (sIg) on both the FL and the tDL, though the immunophenotype of one case of FL could not be definitively determined. In 14 of 15 cases, the surface immunophenotype was preserved; the exception was likely secondary to a class switch from IgM to IgG. In 12 of 13 cases, antiidiotypic monoclonal antibodies prepared against the FL reacted with the paired tDL. Analysis of Ig gene rearrangements in four cases by Southern blot hybridization showed evidence of clonal relationships in all cases though concordance was not seen with all probes tested (C kappa, C lambda, JH, PFL1, and PFL2). In the one case that had a discordant L chain rearrangement, sequence analysis of the L chain demonstrated a common mature B cell origin for both the FL and tDL. To determine whether tDL arose from one or more FL cells, the sequences of the H chain variable genes were analyzed. Individual clones of the V region gene of the FL showed a random distribution of changes throughout the sequence. In contrast, individual clones of the V region gene from tDL shared numerous nonrandom sequence alterations, implying a common single cell origin. In conclusion, tDL is a mature B cell and arises by transformation of a single FL cell.

MeSH Terms
Antibodies, Anti-Idiotypic Antibodies, Monoclonal Base Sequence Blotting, Southern Cell Transformation, Neoplastic/immunology Clone Cells Humans Immunoglobulin Heavy Chains/genetics Immunoglobulin Light Chains/genetics Immunoglobulin Variable Region/genetics Lymphoma, B-Cell/genetics,immunology,pathology Lymphoma, Follicular/genetics,immunology,pathology Lymphoma, Non-Hodgkin/etiology,genetics,immunology Molecular Sequence Data
Chemicals
Antibodies, Anti-Idiotypic Antibodies, Monoclonal Immunoglobulin Heavy Chains Immunoglobulin Light Chains Immunoglobulin Variable Region
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zelenetz A D
Department of Medicine, Stanford Medical School, California 94305.
Chen T T
Levy R
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.173.1.197
Published
1991-01-01
Pages
197-207
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2118744
Subset
IM
Grants
NCI NIH HHS · CA-33399 · United States
NCI NIH HHS · CA-34233 · United States
NCI NIH HHS · K08 CA01396 · United States
External Links
PubMed source DOI: 10.1084/jem.173.1.197 PMC Full Text View journal details
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