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PMID: 19081841 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A central role of abscisic acid in stress-regulated carbohydrate metabolism.

PloS one ·Vol. 3 ·No. 12 ·2008-00-00 ·Pages e3935

Kempa S, Krasensky J, Dal Santo S, Kopka J, Jonak C

Abstract

Abiotic stresses adversely affect plant growth and development. The hormone abscisic acid (ABA) plays a central role in the response and adaptation to environmental constraints. However, apart from the well established role of ABA in regulating gene expression programmes, little is known about its function in plant stress metabolism. Using an integrative multiparallel approach of metabolome and transcriptome analyses, we studied the dynamic response of the model glyophyte Arabidopsis thaliana to ABA and high salt conditions. Our work shows that salt stress induces complex re-adjustment of carbohydrate metabolism and that ABA triggers the initial steps of carbon mobilisation. These findings open new perspectives on how high salinity and ABA impact on central carbohydrate metabolism and highlight the power of iterative combinatorial approaches of non-targeted and hypothesis-driven experiments in stress biology.

MeSH Terms
Abscisic Acid/pharmacology Arabidopsis/drug effects,genetics,physiology Carbohydrate Metabolism/drug effects Gene Expression Regulation, Plant/drug effects Genes, Plant Salinity Sodium Chloride/pharmacology Stress, Physiological/drug effects Time Factors Transcription, Genetic/drug effects
Chemicals
Sodium Chloride Abscisic Acid
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kempa Stefan
Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna, Austria.
Krasensky Julia
Dal Santo Silvia
Kopka Joachim
Jonak Claudia
References (61)
61 references, click to expand
  1. Organic osmolytes as compatible, metabolic and counteracting cytoprotectants in high osmolarity and other stresses.
    J Exp Biol. 2005 Aug;208(Pt 15):2819-30 PMID: 16043587
  2. Light-dependent induction of proline biosynthesis by abscisic acid and salt stress is inhibited by brassinosteroid in Arabidopsis.
    Plant Mol Biol. 2003 Feb;51(3):363-72 PMID: 12602867
  3. Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and other compatible solutes.
    Curr Opin Plant Biol. 2002 Jun;5(3):250-7 PMID: 11960744
  4. Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses.
    Annu Rev Plant Biol. 2006;57:781-803 PMID: 16669782
  5. beta-Amylase induction and the protective role of maltose during temperature shock.
    Plant Physiol. 2004 Jul;135(3):1674-84 PMID: 15247404
  6. A prominent role for the CBF cold response pathway in configuring the low-temperature metabolome of Arabidopsis.
    Proc Natl Acad Sci U S A. 2004 Oct 19;101(42):15243-8 PMID: 15383661
  7. PageMan: an interactive ontology tool to generate, display, and annotate overview graphs for profiling experiments.
    BMC Bioinformatics. 2006 Dec 18;7:535 PMID: 17176458
  8. An update on abscisic acid signaling in plants and more...
    Mol Plant. 2008 Mar;1(2):198-217 PMID: 19825533
  9. Selective mRNA translation coordinates energetic and metabolic adjustments to cellular oxygen deprivation and reoxygenation in Arabidopsis thaliana.
    Plant J. 2008 Dec;56(5):743-55 PMID: 18665916
  10. A Robot-based platform to measure multiple enzyme activities in Arabidopsis using a set of cycling assays: comparison of changes of enzyme activities and transcript levels during diurnal cycles and in prolonged darkness.
    Plant Cell. 2004 Dec;16(12):3304-25 PMID: 15548738
  11. A novel role for abscisic acid emerges from underground.
    Trends Plant Sci. 2006 Sep;11(9):434-9 PMID: 16890475
  12. Perception and transduction of abscisic acid signals: keys to the function of the versatile plant hormone ABA.
    Trends Plant Sci. 2007 Aug;12(8):343-51 PMID: 17629540
  13. Salinity stress adaptation competence in the extremophile Thellungiella halophila in comparison with its relative Arabidopsis thaliana.
    Plant J. 2005 Dec;44(5):826-39 PMID: 16297073
  14. Recent developments in understanding the regulation of starch metabolism in higher plants.
    J Exp Bot. 2004 Oct;55(406):2131-45 PMID: 15361536
  15. The RNA-binding protein FCA is an abscisic acid receptor.
    Nature. 2006 Jan 19;439(7074):290-4 PMID: 16421562
  16. Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray.
    Plant J. 2002 Aug;31(3):279-92 PMID: 12164808
  17. Regulation of osmotic stress-responsive gene expression by the LOS6/ABA1 locus in Arabidopsis.
    J Biol Chem. 2002 Mar 8;277(10):8588-96 PMID: 11779861
  18. Proline accumulation and the adaptation of cultured plant cells to water stress.
    Plant Physiol. 1986 Apr;80(4):938-45 PMID: 16664745
  19. Integrative functional genomics of salt acclimatization in the model legume Lotus japonicus.
    Plant J. 2008 Mar;53(6):973-87 PMID: 18047558
  20. Gene expression profiles during the initial phase of salt stress in rice.
    Plant Cell. 2001 Apr;13(4):889-905 PMID: 11283343
  21. Exploring the temperature-stress metabolome of Arabidopsis.
    Plant Physiol. 2004 Dec;136(4):4159-68 PMID: 15557093
  22. Plant salt tolerance.
    Trends Plant Sci. 2001 Feb;6(2):66-71 PMID: 11173290
  23. MAPMAN: a user-driven tool to display genomics data sets onto diagrams of metabolic pathways and other biological processes.
    Plant J. 2004 Mar;37(6):914-39 PMID: 14996223
  24. RNA interference of Arabidopsis beta-amylase8 prevents maltose accumulation upon cold shock and increases sensitivity of PSII photochemical efficiency to freezing stress.
    Plant J. 2005 Dec;44(5):730-43 PMID: 16297066
  25. The AtGenExpress hormone and chemical treatment data set: experimental design, data evaluation, model data analysis and data access.
    Plant J. 2008 Aug;55(3):526-42 PMID: 18419781
  26. Abscisic acid is an endogenous cytokine in human granulocytes with cyclic ADP-ribose as second messenger.
    Proc Natl Acad Sci U S A. 2007 Apr 3;104(14):5759-64 PMID: 17389374
  27. Non-linear PCA: a missing data approach.
    Bioinformatics. 2005 Oct 15;21(20):3887-95 PMID: 16109748
  28. Abscisic acid signaling through cyclic ADP-ribose in hydroid regeneration.
    J Biol Chem. 2004 Sep 17;279(38):39783-8 PMID: 15252012
  29. Cell signaling during cold, drought, and salt stress.
    Plant Cell. 2002;14 Suppl:S165-83 PMID: 12045276
  30. Transcript and metabolite profiling during cold acclimation of Arabidopsis reveals an intricate relationship of cold-regulated gene expression with modifications in metabolite content.
    Plant J. 2007 Jun;50(6):967-81 PMID: 17461790
  31. The AtGenExpress global stress expression data set: protocols, evaluation and model data analysis of UV-B light, drought and cold stress responses.
    Plant J. 2007 Apr;50(2):347-63 PMID: 17376166
  32. Integration of abscisic acid signalling into plant responses.
    Plant Biol (Stuttg). 2006 May;8(3):314-25 PMID: 16807823
  33. ABSCISIC ACID SIGNAL TRANSDUCTION.
    Annu Rev Plant Physiol Plant Mol Biol. 1998 Jun;49:199-222 PMID: 15012233
  34. PLANT CELLULAR AND MOLECULAR RESPONSES TO HIGH SALINITY.
    Annu Rev Plant Physiol Plant Mol Biol. 2000 Jun;51:463-499 PMID: 15012199
  35. Transcriptome changes for Arabidopsis in response to salt, osmotic, and cold stress.
    Plant Physiol. 2002 Dec;130(4):2129-41 PMID: 12481097
  36. Construction and application of a mass spectral and retention time index database generated from plant GC/EI-TOF-MS metabolite profiles.
    Phytochemistry. 2003 Mar;62(6):887-900 PMID: 12590116
  37. Gamma-aminobutyrate: defense against invertebrate pests?
    Trends Plant Sci. 2006 Sep;11(9):424-7 PMID: 16890474
  38. Uncoupling the effects of abscisic acid on plant growth and water relations. Analysis of sto1/nced3, an abscisic acid-deficient but salt stress-tolerant mutant in Arabidopsis.
    Plant Physiol. 2004 Oct;136(2):3134-47 PMID: 15466233
  39. Water stress enhances beta-amylase activity in cucumber cotyledons.
    J Exp Bot. 2000 Apr;51(345):739-45 PMID: 10938866
  40. Abscisic acid controls calcium-dependent egress and development in Toxoplasma gondii.
    Nature. 2008 Jan 10;451(7175):207-10 PMID: 18185591
  41. The dehydrogenase-mediated recycling of NADPH is a key antioxidant system against salt-induced oxidative stress in olive plants.
    Plant Cell Environ. 2006 Jul;29(7):1449-59 PMID: 17080966
  42. The Arabidopsis LOS5/ABA3 locus encodes a molybdenum cofactor sulfurase and modulates cold stress- and osmotic stress-responsive gene expression.
    Plant Cell. 2001 Sep;13(9):2063-83 PMID: 11549764
  43. [email protected]: the Golm Metabolome Database.
    Bioinformatics. 2005 Apr 15;21(8):1635-8 PMID: 15613389
  44. Leaf starch degradation comes out of the shadows.
    Trends Plant Sci. 2005 Mar;10(3):130-7 PMID: 15749471
  45. Comparative physiology of salt and water stress.
    Plant Cell Environ. 2002 Feb;25(2):239-250 PMID: 11841667
  46. Adaptive response to salt involving carbohydrate metabolism in leaves of a salt-sensitive tomato cultivar.
    Plant Physiol Biochem. 2007 Aug;45(8):551-9 PMID: 17624795
  47. Starch-related alpha-glucan/water dikinase is involved in the cold-induced development of freezing tolerance in Arabidopsis.
    Plant Physiol. 2005 Jun;138(2):837-46 PMID: 15894744
  48. Gene expression and metabolite profiling of Populus euphratica growing in the Negev desert.
    Genome Biol. 2005;6(12):R101 PMID: 16356264
  49. Osmotic regulation of starch synthesis in potato tubers?
    Planta. 1988 Apr;174(1):123-6 PMID: 24221428
  50. GABA in plants: just a metabolite?
    Trends Plant Sci. 2004 Mar;9(3):110-5 PMID: 15003233
  51. Important roles of drought- and cold-inducible genes for galactinol synthase in stress tolerance in Arabidopsis thaliana.
    Plant J. 2002 Feb;29(4):417-26 PMID: 11846875
  52. Highway or byway: the metabolic role of the GABA shunt in plants.
    Trends Plant Sci. 2008 Jan;13(1):14-9 PMID: 18155636
  53. A G protein-coupled receptor is a plasma membrane receptor for the plant hormone abscisic acid.
    Science. 2007 Mar 23;315(5819):1712-6 PMID: 17347412
  54. Plant productivity and environment.
    Science. 1982 Oct 29;218(4571):443-8 PMID: 17808529
  55. beta-Maltose is the metabolically active anomer of maltose during transitory starch degradation.
    Plant Physiol. 2005 Feb;137(2):756-61 PMID: 15665241
  56. The temperature-signaling cascade in sponges involves a heat-gated cation channel, abscisic acid, and cyclic ADP-ribose.
    Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):14859-64 PMID: 11752433
  57. Time-course metabolic profiling in Arabidopsis thaliana cell cultures after salt stress treatment.
    J Exp Bot. 2007;58(3):415-24 PMID: 17118972
  58. A plastid-localized glycogen synthase kinase 3 modulates stress tolerance and carbohydrate metabolism.
    Plant J. 2007 Mar;49(6):1076-90 PMID: 17319843
  59. Starch degradation.
    Annu Rev Plant Biol. 2005;56:73-98 PMID: 15862090
  60. The Mg-chelatase H subunit is an abscisic acid receptor.
    Nature. 2006 Oct 19;443(7113):823-6 PMID: 17051210
  61. Metabolite fingerprinting: detecting biological features by independent component analysis.
    Bioinformatics. 2004 Oct 12;20(15):2447-54 PMID: 15087312
Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0003935
Published
2008-00-00
Epub
2008-00-12
Pages
e3935
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC2593778
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pone.0003935 PMC Full Text View journal details
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