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PMID: 1937770 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and molecular characterization of spontaneously occurring cytolysin-negative mutants of Actinobacillus pleuropneumoniae serotype 7.

Infection and immunity ·Vol. 59 ·No. 11 ·1991-11-00 ·Pages 4110-6

Anderson C, Potter AA, Gerlach GF

Abstract

Actinobacillus pleuropneumoniae serotype 7 strains are shown to spontaneously lose cytolytic activity with a frequency of approximately 10(-4). The phenotypic change is associated with the loss of approximately 8.5 kbp of chromosomal DNA. A genomic fragment encoding the cytolysin and its flanking sequences was cloned and characterized. Also, the corresponding truncated fragment was cloned from a spontaneous mutant. Comparison of the two clones allowed the definition of the excision site. The ends of the excised fragment are composed of 1,201 bp long direct identical repeats, possibly facilitating the genotypic change by homologous recombination. In accordance with this hypothesis, one repeat is conserved in the spontaneous mutant. Each repeat contains one open reading frame preceded by a Shine-Dalgarno consensus sequence, and the ends of each repeat contain 26-bp complementary sequences with four mismatches.

Related Genes
cytA
MeSH Terms
Actinobacillus pleuropneumoniae/genetics Base Sequence Blotting, Southern Chromosome Deletion Cloning, Molecular Cytotoxins/genetics DNA Mutational Analysis DNA, Bacterial/genetics Genes, Bacterial Molecular Sequence Data Mutation Repetitive Sequences, Nucleic Acid Restriction Mapping
Chemicals
Cytotoxins DNA, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Anderson C
Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada.
Potter A A
Gerlach G F
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33 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.59.11.4110-4116.1991
Published
1991-11-00
Pages
4110-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC259004
Subset
IM
Databases
GENBANK
M74588
External Links
PubMed source DOI: 10.1128/iai.59.11.4110-4116.1991 PMC Full Text View journal details
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