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PMID: 1942257 Published · ppublish English Journal Article

Functional mapping of the human immunodeficiency virus type 1 Rev RNA binding domain: new insights into the domain structure of Rev and Rex.

Journal of virology ·Vol. 65 ·No. 12 ·1991-12-00 ·Pages 7051-5

Böhnlein E, Berger J, Hauber J

Abstract

Expression of human immunodeficiency virus type 1 (HIV-1) structural proteins requires the direct interaction of the viral trans-activator protein Rev with its cis-acting RNA sequence (Rev-response element [RRE]). A stretch of 14 amino acid residues of the 116-amino-acid Rev protein is sufficient to impose nucleolar localization onto a heterologous protein. Our results demonstrated that these same amino acid residues confer Rev-specific RRE binding to the heterologous human T-cell leukemia virus type I Rex protein. In addition, our results indicated that amino acids distinct from the nuclear localization signal are important for Rex-specific RRE RNA binding.

Related Genes
Rev Rex
MeSH Terms
Amino Acid Sequence Animals Binding Sites Cell Line Gene Products, rev/genetics,metabolism Gene Products, rex/genetics,metabolism Genes, pX Genes, rev HIV-1/genetics,metabolism Kinetics Molecular Sequence Data RNA, Viral/genetics,metabolism Transfection rev Gene Products, Human Immunodeficiency Virus
Chemicals
Gene Products, rev Gene Products, rex RNA, Viral rev Gene Products, Human Immunodeficiency Virus
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Böhnlein E
SANDOZ Research Institute, Vienna, Austria.
Berger J
Hauber J
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43 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.65.12.7051-7055.1991
Published
1991-12-00
Pages
7051-5
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC250826
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.65.12.7051-7055.1991 PMC Full Text View journal details
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