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PMID: 19602544 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Overexpression of the soybean GmERF3 gene, an AP2/ERF type transcription factor for increased tolerances to salt, drought, and diseases in transgenic tobacco.

Journal of experimental botany ·Vol. 60 ·No. 13 ·2009-00-00 ·Pages 3781-96

Zhang G, Chen M, Li L, Xu Z, Chen X, Guo J, Ma Y

Abstract

A new member of the AP2/ERF transcription factor family, GmERF3, was isolated from soybean. Sequence analysis showed that GmERF3 contained an AP2/ERF domain of 58 amino acids and two putative nuclear localization signal (NLS) domains. It belonged to a group IV protein in the ERF (ethylene response factor) subfamily as typified by a conserved N-terminal motif [MCGGAI(I/L)]. Expression of GmERF3 was induced by treatments with high salinity, drought, abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), ethylene (ET), and soybean mosaic virus (SMV), whereas there was no significant GmERF3 mRNA accumulation under cold stress treatment. GmERF3 could bind to the GCC box and DRE/CRT element, and was targeted to the nucleus when transiently expressed in onion epidermal cells. The GmERF3 protein fused to the GAL4 DNA-binding domain to activate transcription of reporter genes in yeast. Ectopic expression of the GmERF3 gene in transgenic tobacco plants induced the expression of some PR genes and enhanced resistance against infection by Ralstonia solanacearum, Alternaria alternata, and tobacco mosaic virus (TMV), and gave tolerance to high salinity and dehydration stresses. Furthermore, overexpression of GmERF3 in transgenic tobacco led to higher levels of free proline and soluble carbohydrates compared to wild-type plants under drought conditions. The overall results suggested that GmERF3 as an AP2/ERF transcription factor may play dual roles in response to biotic and abiotic stresses in plants.

MeSH Terms
Alternaria/physiology Amino Acid Sequence Droughts Gene Expression Regulation, Plant Immunity, Innate Molecular Sequence Data Plant Diseases/microbiology,virology Plants, Genetically Modified/genetics,immunology,physiology Ralstonia/physiology Salt Tolerance Sequence Alignment Soybean Proteins/chemistry,genetics,metabolism Soybeans/chemistry,genetics,metabolism Tobacco/genetics,immunology,physiology Tobacco Mosaic Virus/physiology Transcription Factors/chemistry,genetics,metabolism
Chemicals
Soybean Proteins Transcription Factors
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Zhang Gaiyun
The National Key Facility for Crop Genetic Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Chen Ming
Li Liancheng
Xu Zhaoshi
Chen Xueping
Guo Jiaming
Ma Youzhi
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Article Info
Journal
Journal of experimental botany
Abbr.
J Exp Bot
ISSN
1460-2431
DOI
10.1093/jxb/erp214
Published
2009-00-00
Epub
2009-00-14
Pages
3781-96
Language
English
Region
England
NLM ID
9882906
PMCID
PMC2736888
Subset
IM
External Links
PubMed source DOI: 10.1093/jxb/erp214 PMC Full Text View journal details
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