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PMID: 1970114 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The fimD gene required for cell surface localization of Escherichia coli type 1 fimbriae.

Molecular & general genetics : MGG ·Vol. 220 ·No. 2 ·1990-01-00 ·Pages 334-8

Klemm P, Christiansen G

Abstract

The fimD gene of Escherichia coli K12 was shown to be necessary for surface localization of type 1 fimbriae, since deletion of the gene resulted in a virtually bald phenotype. The FimD protein was found to be located in the outer membrane. Expressed alone, this protein had a very deleterious effect on cell growth. The DNA sequence of the fimD gene was determined; the corresponding amino acid sequence of the FimD protein was compared with those of the PapC and FaeD proteins. A deletion derivative of FimD gave clues as to which parts of the protein were necessary for outer membrane integration.

MeSH Terms

Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Cell Membrane/analysis,ultrastructure Escherichia coli/genetics,growth & development,ultrastructure Fimbriae, Bacterial/analysis,ultrastructure Genes, Bacterial Genetic Complementation Test Microscopy, Electron Molecular Sequence Data Phenotype Restriction Mapping

Chemicals

Bacterial Proteins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Klemm P

Department of Microbiology, Technical University of Denmark, Lyngby.

Christiansen G

References (14)

14 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00260505

Published

1990-01-00

Pages

334-8

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00260505

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The fimD gene required for cell surface localization of Escherichia coli type 1 fimbriae.

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