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PMID: 2863734 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The fim genes responsible for synthesis of type 1 fimbriae in Escherichia coli, cloning and genetic organization.

Molecular & general genetics : MGG ·Vol. 199 ·No. 3 ·1985-00-00 ·Pages 410-4

Klemm P, Jørgensen BJ, van Die I, de Ree H, Bergmans H

Abstract

The genes responsible for the expression of type 1 fimbriae, produced by the majority of E. coli strains, have been cloned from an E. coli K12 strain. The "passenger" DNA from an initial cosmid clone was reduced in size and subcloned in pACYC184 and pBR322 vectors. A DNA fragment of around 8 kbp was found to be required for the biosynthesis of type 1 fimbriae. This was further studied by transposon-mediated insertional inactivation and by BAL31-mediated deletions. Four genes, designated fimA, B, C, and D were found to be involved in the synthesis of the fimbriae. They encoded proteins that in their processed form appeared with apparent molecular weights of 16.5 kd, 23 kd, 26 kd, and 89 kd, the 16.6 kd polypeptide being the fimbrial subunit. The order to the genes was found to be: fimB, fimA, fimC, and fimD, organized in three transcriptional units.

MeSH Terms
Animals Bacterial Outer Membrane Proteins/genetics Base Sequence Cloning, Molecular DNA Restriction Enzymes Escherichia coli/genetics,ultrastructure Fimbriae Proteins Fimbriae, Bacterial/physiology Genes, Bacterial Genetic Complementation Test Guinea Pigs Hemagglutination Molecular Weight Plasmids
Chemicals
Bacterial Outer Membrane Proteins Fimbriae Proteins DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Klemm P
Jørgensen B J
van Die I
de Ree H
Bergmans H
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28 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330751
Published
1985-00-00
Pages
410-4
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00330751
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