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PMID: 342904 Published · ppublish English Journal Article

Origin of replication, oriC, of the Escherichia coli chromosome: mapping of genes relative to R.EcoRI cleavage sites in the oriC region.

Molecular & general genetics : MGG ·Vol. 158 ·No. 1 ·1977-12-14 ·Pages 101-9

von Meyenburg K, Hansen FG, Nielsen LD, Jørgensen P

Abstract

A precise genetic-physical map of the tna-ilv region at 82 min on the genetic map of E. coli is obtained through deletion mapping and analysis by restriction endonuclease EcoRI of plasmids, derived from an F' carrying the genes between aroE and ilv. A locus, designated het, which in its diploid state results in slow growth and heterogeneity of cell size due to distorted cell division, maps between bglB and asn, 30-45 kb counterclockwise of ilv. The pattern of R.EcoRI cleavage sites in the het region is identical with the pattern obtained by Marsh and Worcel (1977) who analyzed DNA labeled preferentially in the region of the DNA replication origin (oriC). We suggest that oriC is identical with the het site and that it can be allocated to a position 32 kb counterclockwise of the ilv operon.

MeSH Terms
Chromosome Mapping Chromosomes, Bacterial DNA Replication Escherichia coli/genetics F Factor Genes Plasmids
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
von Meyenburg K
Hansen F G
Nielsen L D
Jørgensen P
References (31)
31 references, click to expand
  1. Origin and sequence of chromosome replication in Escherichia coli.
    J Mol Biol. 1972 Oct 14;70(3):549-66 PMID: 4563262
  2. More precise mapping of the replication origin in Escherichia coli K-12.
    J Bacteriol. 1974 Oct;120(1):1-5 PMID: 4607435
  3. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  4. Evidence for the bidirectional replications of the Escherichia coli chromosome.
    Nat New Biol. 1971 Aug 4;232(31):137-40 PMID: 4937091
  5. A ribosomal RNA gene, rrnC, of Escherichia coli, mapped by specialized transducing lambdadilv and lambda drbs phages.
    Mol Gen Genet. 1978 Jul 11;163(2):223-8 PMID: 355851
  6. A ribosomal RNA gene of Escherichia coli (rrnD) on lamnda daro E specialized transducing phages.
    Mol Gen Genet. 1976 Aug 2;146(3):303-7 PMID: 794695
  7. Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis.
    J Virol. 1974 Nov;14(5):1235-44 PMID: 4372397
  8. A DNA fragment containing the origin of replication of the Escherichia coli chromosome.
    Proc Natl Acad Sci U S A. 1977 Jul;74(7):2720-4 PMID: 268621
  9. A simple method for the preparation of large quantities of pure plasmid DNA.
    Biochim Biophys Acta. 1975 Apr 2;383(4):457-63 PMID: 1092355
  10. Molecular cloning of DNA from F sex factor of Escherichia coli K-12.
    Proc Natl Acad Sci U S A. 1976 Jan;73(1):64-8 PMID: 1108020
  11. cis-Dominant, transfer-deficient mutants of the Escherichia coli K-12 F sex factor.
    J Bacteriol. 1976 Jan;125(1):233-47 PMID: 1107307
  12. Rapid mapping of conditional and auxotrophic mutations in Escherichia coli K-12.
    J Bacteriol. 1973 Feb;113(2):798-812 PMID: 4570607
  13. Electron microscope study of a plasmid chimera containing the replication region of the Escherichia coli F plasmid.
    J Bacteriol. 1976 Aug;127(2):988-97 PMID: 783125
  14. Cloning, isolation, and characterization of replication regions of complex plasmid genomes.
    Proc Natl Acad Sci U S A. 1975 Jun;72(6):2242-6 PMID: 1094460
  15. Novel F prime factors able to replicate in Escherichia coli Hfr strains.
    Proc Natl Acad Sci U S A. 1976 Jan;73(1):198-202 PMID: 1108015
  16. Bidirectional growth of the E. coli chromosome.
    Nat New Biol. 1973 Apr 4;242(118):130-2 PMID: 4573478
  17. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.
    Proc Natl Acad Sci U S A. 1967 May;57(5):1514-21 PMID: 5231757
  18. Strains of Escherichia coli diploid for the chromosomal origin of DNA replication.
    Mol Gen Genet. 1975 Dec 30;143(1):105-11 PMID: 765740
  19. Escherichia coli K-12 F-prime factors, old and new.
    Bacteriol Rev. 1972 Dec;36(4):587-607 PMID: 4568765
  20. Method for the isolation of the replication region of a bacterial replicon: construction of a mini-F'kn plasmid.
    J Bacteriol. 1976 Aug;127(2):982-7 PMID: 783124
  21. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.
    Biochem J. 1967 Jan;102(1):251-7 PMID: 5339944
  22. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors.
    J Mol Biol. 1972 Nov 14;71(2):471-97 PMID: 4564488
  23. Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants.
    J Bacteriol. 1971 May;106(2):529-38 PMID: 4929865
  24. F 1 sex factor of Escherichia coli. Size and purification in the form of a strand-specific relaxation complex of supercoiled deoxyribonucleic acid and protein.
    Biochemistry. 1971 Dec 21;10(26):4975-80 PMID: 4944065
  25. Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid.
    J Bacteriol. 1975 Jan;121(1):354-62 PMID: 1090574
  26. Recalibrated linkage map of Escherichia coli K-12.
    Bacteriol Rev. 1976 Mar;40(1):116-67 PMID: 773363
  27. Pedigrees of some mutant strains of Escherichia coli K-12.
    Bacteriol Rev. 1972 Dec;36(4):525-57 PMID: 4568763
  28. Replication of Fpoh+ plasmid in mafA mutants of Escherichia coli defective in plasmid maintenance.
    Mol Gen Genet. 1977 Apr 29;152(3):211-7 PMID: 327277
  29. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. V. ilv+ Deletion mutants of F14.
    J Mol Biol. 1974 Nov 15;89(4):585-97 PMID: 4615160
  30. Bidirection replication from a unique origin in a mini-F plasmid.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):1138-41 PMID: 322144
  31. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. VII. Mapping the ribosomal RNA genes of plasmid F14.
    J Mol Biol. 1974 Nov 15;89(4):619-29 PMID: 4615162
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00455124
Published
1977-12-14
Pages
101-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00455124
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