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PMID: 197266 Published · ppublish English Journal Article

Simian virus 40-specific ribosome-binding proteins induced by a nondefective adenovirus 2-simian virus 40 hybrid.

Journal of virology ·Vol. 23 ·No. 3 ·1977-09-00 ·Pages 692-9

Jay G, Jay FT, Friedman RM, Levine AS

Abstract

We have studied the intracellular distribution of the two simian virus 40-specific proteins, with apparent molecular weights of 56,000 and 42,000, detectable in human KB cells infected by a nondefective adenovirus 2-simian virus 40 hybrid, Ad2+ND2. After a 20-min pulse of [35S]methionine, about two-thirds of the newly synthesized 56K protein and one-third of the 42K protein were found localized on the plasma membrane. The remainder of each protein was found in the cytoplasm, whereas the nuclear fraction was virtually free of either component. A significant portion of both proteins present in the cytoplasmic fraction was complexed to the 40S ribosomal subunits and was not removed by treatment with 0.5 M KCl. Moreover, the portion that was found free in the cytoplasm could bind preferentially and quantitatively to purified 40S ribosomes in vitro, leading us to propose that these simian virus 40 proteins may act as translational control elements in cells.

MeSH Terms
Adenoviruses, Human/growth & development Cell Line Humans Hybridization, Genetic Molecular Weight Peptides/analysis Protein Binding Ribosomes/metabolism Simian virus 40/analysis,growth & development,metabolism Viral Proteins/analysis,metabolism
Chemicals
Peptides Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jay G
Jay F T
Friedman R M
Levine A S
References (31)
31 references, click to expand
  1. NEW SURFACE ANTIGEN IN CELLS TRANSFORMED BY SIMIAN PAPOVAVIRUS SV40.
    Proc Soc Exp Biol Med. 1965 Jul;119:896-901 PMID: 14330903
  2. SYNTHESIS OF SV40 TUMOR ANTIGEN DURING REPLICATION OF SIMIAN PAPOVAVIRUS (SV40).
    Proc Natl Acad Sci U S A. 1964 Nov;52:1138-42 PMID: 14231432
  3. ENHANCEMENT OF ADENOVIRUS GROWTH IN AFRICAN GREEN MONKEY KIDNEY CELL CULTURES BY SV40.
    Proc Soc Exp Biol Med. 1964 May;116:187-90 PMID: 14200102
  4. A SPECIFIC COMPLEMENT-FIXING ANTIGEN PRESENT IN SV40 TUMOR AND TRANSFORMED CELLS.
    Proc Natl Acad Sci U S A. 1963 Dec;50:1148-56 PMID: 14096190
  5. IMMUNITY IN HAMSTERS TO CELLS TRANSFORMED IN VITRO AND IN VIVO BY SV40. TESTS FOR ANTIGENIC RELATIONSHIP AMONG THE PAPOVAVIRUSES.
    J Immunol. 1963 Nov;91:604-13 PMID: 14089763
  6. Synthesis, transport, and morphogenesis of type adenovirus capsid proteins.
    J Virol. 1970 Mar;5(3):338-52 PMID: 5461888
  7. DNA-binding proteins specific for cells infected by adenovirus.
    Nat New Biol. 1973 Dec 12;246(154):170-4 PMID: 4202226
  8. Genetic evidence for SV40 gene function in enhancement of replication of human adenovirus in simian cells.
    Nature. 1974 Apr 12;248(449):590-2 PMID: 4363086
  9. Conditional lethal mutants of adenovirus 2-simian virus 40 hybrids. I. Host range mutants of Ad2+ND1.
    J Virol. 1974 Jun;13(6):1237-44 PMID: 4364898
  10. An SV40-induced initiation factor for protein synthesis concerned with the regulation of permissiveness.
    Nature. 1974 Dec 20;252(5485):649-53 PMID: 4373655
  11. Synthesis of viral ribonucleic acid during restricted adenovirus infection.
    J Virol. 1972 Aug;10(2):220-7 PMID: 4342240
  12. Studies of the mechanism of enhancement of human adenovirus infection in monkey cells by simian virus 40.
    J Virol. 1972 Aug;10(2):211-9 PMID: 4342239
  13. Studies on nondefective adenovirus-simian virus 40 hybrid viruses. I. A newly characterized simian virus 40 antigen induced by the Ad2+ND 1 virus.
    J Virol. 1971 Feb;7(2):189-97 PMID: 4329396
  14. Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. VII. Characterization of the simian virus 40 RNA species induced by five nondefective hybrid viruses.
    J Virol. 1973 May;11(5):672-81 PMID: 4350712
  15. Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. V. Isolation of additional hybrids which differ in their simian virus 40-specific biological properties.
    J Virol. 1973 May;11(5):655-64 PMID: 4350710
  16. Mapping of simian virus 40 early functions on the viral chromosome.
    J Virol. 1973 Sep;12(3):653-8 PMID: 4355862
  17. Viral-induced enzymes and the problem of viral oncogenesis.
    Adv Cancer Res. 1968;11:73-221 PMID: 4306735
  18. Induction of SV40 transplantation antigen (TrAg) during the lytic cycle.
    Virology. 1970 Nov;42(3):688-98 PMID: 4320667
  19. Simian virus to (SV40) tumor-specific proteins in nucleus and plasma membrane of HeLa cells infected by adenovirus 2-SV40 hybrid virus Ad2+ND2.
    Proc Natl Acad Sci U S A. 1976 Jul;73(7):2505-9 PMID: 59927
  20. The polypeptides of adenovirus-infected cells.
    J Gen Virol. 1972 Apr;15(1):45-57 PMID: 4623637
  21. The identification of prereplicative bacteriophage T4 proteins.
    J Biol Chem. 1973 Aug 10;248(15):5499-501 PMID: 4588684
  22. Enhancement of the replication of human adenovirus in simian cells by a series of temperature-sensitive mutants of simian virus 40.
    Virology. 1975 Jun;65(2):579-82 PMID: 165626
  23. The alteration of ribosomes for mRNA selection concerned with adenovirus growth in SV40-infected simian cells.
    Virology. 1975 Sep;67(1):85-93 PMID: 170741
  24. Simian virus 40-specific proteins in HeLa cells infected with nondefective adenovirus 2-simian virus 40 hybrid viruses.
    J Virol. 1975 Nov;16(5):1236-47 PMID: 171451
  25. Block to multiplication of adenovirus serotype 2 in monkey cells.
    J Virol. 1975 Dec;16(6):1650-68 PMID: 172661
  26. Amount of viral DNA in the genome of cells transformed by adenovirus type 2.
    J Mol Biol. 1973 Jan;73(1):125-30 PMID: 4734785
  27. Processing of adenovirus 2-induced proteins.
    J Virol. 1973 Aug;12(2):241-52 PMID: 4747985
  28. Nonribosomal proteins associated with eukaryotic native small ribosomal subunits.
    Proc Natl Acad Sci U S A. 1975 Sep;72(9):3392-6 PMID: 1059126
  29. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
    Eur J Biochem. 1975 Aug 15;56(2):335-41 PMID: 1175627
  30. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  31. Synthesis and assembly of adenovirus 2. I. Polypeptide synthesis, assembly of capsomeres, and morphogenesis of the virion.
    Virology. 1969 Dec;39(4):682-94 PMID: 5358824
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.23.3.692-699.1977
Published
1977-09-00
Pages
692-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515881
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.23.3.692-699.1977 PMC Full Text View journal details
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