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PMID: 1979856 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Genomic analysis of the major bovine milk protein genes.

Nucleic acids research ·Vol. 18 ·No. 23 ·1990-12-11 ·Pages 6935-42

Threadgill DW, Womack JE

Abstract

The genomic arrangement of the major bovine milk protein genes has been determined using a combination of physical mapping techniques. The major milk proteins consist of the four caseins, alpha s1 (CASAS1), alpha s2 (CASAS2), beta (CASB), and kappa (CASK), as well as the two major whey proteins, alpha-lactalbumin (LALBA) and beta-lactoglobulin (LGB). A panel of bovine X hamster hybrid somatic cells analyzed for the presence or absence of bovine specific restriction fragments revealed the genes coding for the major milk proteins to reside on three chromosomes. The four caseins were assigned to syntenic group U15 and localized to bovine chromosome 6 at q31-33 by in situ hybridization. LALBA segregated with syntenic group U3, while LGB segregated with U16. Pulsed-field gel electrophoresis confirmed genetic mapping results indicating tight linkage of the casein genes. The four genes reside on less than 200 kb of DNA in the order CASAS1-CASB-CASAS2-CASK. Multiple restriction fragment length polymorphisms were also found at the six loci in three breeds of cattle.

Related Genes
CASAS1 CASAS2 CASB CASK
MeSH Terms
Animals Caseins/genetics Cattle Cricetinae Genes Genetic Linkage Hybrid Cells Lactalbumin/genetics Lactoglobulins/genetics Nucleic Acid Hybridization Polymorphism, Restriction Fragment Length Restriction Mapping
Chemicals
Caseins Lactoglobulins Lactalbumin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Threadgill D W
Department of Veterinary Pathology, Texas A&M University, College Station 77843.
Womack J E
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.23.6935
Published
1990-12-11
Pages
6935-42
Language
English
Region
England
NLM ID
0411011
PMCID
PMC332753
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/18.23.6935 PMC Full Text View journal details
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