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PMID: 1979945 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

An autoregulatory enhancer element of the Drosophila homeotic gene Deformed.

The EMBO journal ·Vol. 9 ·No. 13 ·1990-12-00 ·Pages 4287-97

Bergson C, McGinnis W

Abstract

The stable determination of different anterior-posterior regions of the Drosophila embryo is controlled by the persistent expression of homeotic selector genes. One mechanism that has been proposed to explain the persistent expression of the homeotic gene Deformed is an autoactivation circuit that would be used once Deformed expression had been established by earlier acting patterning genes. Here we show that a large cis-regulatory element mapping approximately 5 kb upstream of the Deformed transcription start has the properties predicted for a Deformed autoregulatory enhancer. This element provides late, spatially localized expression in the epidermal cells of the maxillary and mandibular segments which is wholly dependent upon endogenous Deformed function. In addition, the autoregulatory enhancer can be activated ectopically in embryos and in imaginal disc cells by ectopic expression of Deformed protein. Deletion analysis of the autoregulatory element indicates that it contains compartment specific sub-elements similar to those of other homeotic loci.

Related Genes
Dfd
MeSH Terms
Animals Base Sequence DNA-Binding Proteins/biosynthesis,genetics Drosophila/embryology,genetics Drosophila Proteins Enhancer Elements, Genetic Epidermis/metabolism,ultrastructure Fluorescent Antibody Technique Gene Expression Regulation Genes, Homeobox Homeodomain Proteins Lac Operon Mandible/metabolism,ultrastructure Maxilla/metabolism,ultrastructure Molecular Sequence Data Molecular Weight
Chemicals
DNA-Binding Proteins Dfd protein, Drosophila Drosophila Proteins Homeodomain Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bergson C
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511.
McGinnis W
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-12-00
Pages
4287-97
Language
English
Region
England
NLM ID
8208664
PMCID
PMC552211
Subset
IM
Grants
NIGMS NIH HHS · P01GM39813 · United States
External Links
PubMed source PMC Full Text View journal details
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