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PMID: 19843612 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

Sequence-non-specific effects of RNA interference triggers and microRNA regulators.

Nucleic acids research ·Vol. 38 ·No. 1 ·2010-01-00 ·Pages 1-16

Olejniczak M, Galka P, Krzyzosiak WJ

Abstract

RNA reagents of diverse lengths and structures, unmodified or containing various chemical modifications are powerful tools of RNA interference and microRNA technologies. These reagents which are either delivered to cells using appropriate carriers or are expressed in cells from suitable vectors often cause unintended sequence-non-specific immune responses besides triggering intended sequence-specific silencing effects. This article reviews the present state of knowledge regarding the cellular sensors of foreign RNA, the signaling pathways these sensors mobilize and shows which specific features of the RNA reagents set the responsive systems on alert. The representative examples of toxic effects caused in the investigated cell lines and tissues by the RNAs of specific types and structures are collected and may be instructive for further studies of sequence-non-specific responses to foreign RNA in human cells.

MeSH Terms
Base Sequence Gene Expression Profiling Humans MicroRNAs/metabolism Proteomics RNA/chemistry RNA Interference RNA, Small Interfering/chemistry,metabolism RNA-Binding Proteins/chemistry Signal Transduction
Chemicals
MicroRNAs RNA, Small Interfering RNA-Binding Proteins RNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Olejniczak Marta
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.
Galka Paulina
Krzyzosiak Wlodzimierz J
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkp829
Published
2010-01-00
Epub
2009-00-20
Pages
1-16
Language
English
Region
England
NLM ID
0411011
PMCID
PMC2800214
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/gkp829 PMC Full Text View journal details
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