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PMID: 1986214 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of wild-type p53 is not compatible with continued growth of p53-negative tumor cells.

Molecular and cellular biology ·Vol. 11 ·No. 1 ·1991-01-00 ·Pages 1-11

Johnson P, Gray D, Mowat M, Benchimol S

Abstract

Inactivation of the cellular p53 gene is a common feature of Friend virus-induced murine erythroleukemia cell lines and may represent a necessary step in the progression of this disease. As well, frequent loss or mutation of p53 alleles in diverse human tumors is consistent with the view of p53 as a tumor suppressor gene. To examine the significance of p53 gene inactivation in tumorigenesis, we have attempted to express transfected wild-type p53 in three p53-negative tumor cell lines: murine DP16-1 Friend erythroleukemia cells, human K562 cells, and SKOV-3 cells. We found that aberrant p53 proteins, which differ from wild-type p53 by a single amino acid substitution, were expressed stably in these cells, whereas wild-type p53 expression was not tolerated. The inability of p53-negative tumor cell lines to support long-term expression of wild-type p53 protein is consistent with the view that p53 is a tumor suppressor gene.

MeSH Terms
Animals Base Sequence Blotting, Northern Blotting, Southern Cell Division Cell Transformation, Neoplastic/genetics DNA, Neoplasm/genetics Gene Expression Genes, Tumor Suppressor Mice Molecular Sequence Data Oligonucleotides RNA, Neoplasm/genetics Transfection Tumor Cells, Cultured Tumor Suppressor Protein p53/genetics,physiology
Chemicals
DNA, Neoplasm Oligonucleotides RNA, Neoplasm Tumor Suppressor Protein p53
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Johnson P
Ontario Cancer Institute, University of Toronto, Canada.
Gray D
Mowat M
Benchimol S
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.1.1-11.1991
Published
1991-01-00
Pages
1-11
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359576
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.11.1.1-11.1991 PMC Full Text View journal details
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