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PMID: 1986236 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Xenopus B1 factor is closely related to the mammalian activator USF and is implicated in the developmental regulation of TFIIIA gene expression.

Molecular and cellular biology ·Vol. 11 ·No. 1 ·1991-01-00 ·Pages 412-24

Kaulen H, Pognonec P, Gregor PD, Roeder RG

Abstract

The Xenopus laevis TFIIIA promoter contains a motif that has been implicated in promoter activation in late-stage oocytes and contains the sequence (-269) CACGTG (-264). A cDNA encoding a protein (B1) that binds to this element has been cloned from X. laevis and Xenopus borealis ovarian cDNA libraries. We show that this protein is a member of the helix-loop-helix family of regulatory proteins and contains 80% sequence identity with the human adenovirus major late transcription factor (MLTF or USF). A survey of B1 protein expression during oogenesis and embryogenesis revealed both oocyte-specific and somatic cell-specific B1 protein-DNA complexes. Immunological data, RNA blot analysis, and proteolytic clipping band shift assays indicated that these complexes most likely represent altered forms of a single B1 polypeptide. Implications for TFIIIA gene regulation during development are discussed.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern Cloning, Molecular DNA/genetics DNA-Binding Proteins/genetics Gene Expression Regulation Genes Molecular Sequence Data Oligonucleotides/chemistry Oocytes/physiology Polymerase Chain Reaction Promoter Regions, Genetic Protein Processing, Post-Translational RNA, Messenger/genetics Regulatory Sequences, Nucleic Acid Transcription Factor TFIIIA Transcription Factors/genetics,physiology Upstream Stimulatory Factors Xenopus/genetics Xenopus laevis/embryology,physiology
Chemicals
B1 factor, Xenopus DNA-Binding Proteins Oligonucleotides RNA, Messenger Transcription Factor TFIIIA Transcription Factors Upstream Stimulatory Factors DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kaulen H
Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021-6399.
Pognonec P
Gregor P D
Roeder R G
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.1.412-424.1991
Published
1991-01-00
Pages
412-24
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359642
Subset
IM
Grants
NIAID NIH HHS · AI 27397 · United States
NCI NIH HHS · CA-42567 · United States
NIAID NIH HHS · F32AI7696 · United States
Databases
GENBANK
D00651, D00652, D00653, D00654, M63663, M63664, M64825, M64826, M64827, S43885
External Links
PubMed source DOI: 10.1128/mcb.11.1.412-424.1991 PMC Full Text View journal details
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